FICZ (6-Formylindolo[3,2-b]carbazole)

製品コードS9682 バッチS968201

化学情報

	Synonyms	6-Formylindolo[3,2-b]carbazole	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₁₉H₁₂N₂O
	分子量	284.31	CAS No.	172922-91-7
Solubility (25°C)*	体外	DMSO	57 mg/mL (200.48 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	FICZ (6-Formylindolo[3,2-b]carbazole) は、AHRによって制御されるチトクロームP4501酵素によって効率的に代謝される、強力なアリール炭化水素受容体 (AhR) アゴニストです。
in vitro	In vitro studies in cells show that CYP1 inhibition in the presence of FICZ results in enhanced AHR activation, suggesting that this compound accumulates in the cell when its metabolism is blocked. It significantly upregulates the gene expression of filaggrin in both HaCaT cells and NHEKs in an AHR-dependent manner, but does not affect the gene expression of other barrier-related proteins.
in vivo	FICZ improves the atopic dermatitis-like skin inflammation, clinical scores, and transepidermal water loss in NC/Nga mice compared with those of control mice. On histology, this compound significantly reduces the epidermal and dermal thickness as well as the number of mast cells. Topical application also significantly reduces the gene expression of Il22.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Human immortalized HaCaT cells, normal human epidermal keratinocytes (NHEKs)
	濃度	1 nM, 10 nM, 100 nM
	反応時間	1 h, 3 h, 5 h
	実験の流れ	HaCaTcells or NHEKs (1×10⁴ cells/well) are platedon an 8-well μ-slide for 48 h, and treated with or without FICZ for 3 or 1 h, respectively. Then, the cells are washed with phosphate-buffered saline (PBS), fixed with acetone for 10 min, and blocked with 5% (w/v) bovine serum albumin in PBS for 30 min. The cells are then incubated with primary rabbit anti-AHR antibody (1:100) overnight at 4 ℃. Specific binding is detected using a horseradish peroxidase-conjugated goat anti-rabbit anti-body followed by tyramide labeling with green-fluorescent Alexa Fluor 488 for 1 h at room temperature in accordance with the manufacturer's instructions.
動物実験	動物モデル	9–10 week-old female NC/Nga mice
	投薬量	100 mg/affected area
	投与方法	Topical

参考

https://pubmed.ncbi.nlm.nih.gov/27112072/
https://pubmed.ncbi.nlm.nih.gov/29500077/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Loss of aryl hydrocarbon receptor reduces pancreatic tumor growth by increasing immune cell infiltration [ Biochem Pharmacol, 2025, 236:116872]	PubMed: 40090596
Loss of Parp7 increases type I interferon signalling and reduces pancreatic tumour growth by enhancing immune cell infiltration [ Front Immunol, 2024, 15:1513595]	PubMed: 39867896
Tubular aryl hydratocarbon receptor upregulates EZH2 to promote cellular senescence in cisplatin-induced acute kidney injury [ Cell Death Dis, 2023, 14(1):18]	PubMed: 36635272
Intestinal Flora-Derived Kynurenic Acid Protects Against Intestinal Damage Caused by Candida albicans Infection via Activation of Aryl Hydrocarbon Receptor [ Front Microbiol, 2022, 13:934786]	PubMed: 35923391
Novel Pentapeptide Derived from Chicken by-Product Ameliorates DSS-Induced Colitis by Enhancing Intestinal Barrier Function via AhR-Induced Src Inactivation [ J Agric Food Chem, 2020, 10.1021/acs.jafc.0c06319]	PubMed: 33210912

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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