FICZ

製品コードS9682 バッチS968201

印刷

化学情報

Synonyms

6-Formylindolo[3,2-b]carbazole

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₁₉H₁₂N₂O

分子量

284.31

CAS No.

172922-91-7

Solubility (25°C)*

体外

DMSO

57 mg/mL (200.48 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O

1.5mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 30 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	FICZ (6-Formylindolo[3,2-b]carbazole) is a potent agonist of aryl hydrocarbon receptor (AhR) that is efficiently metabolized by AHR-regulated cytochrome P4501 enzymes.
in vitro	In vitro studies in cells show that CYP1 inhibition in the presence of FICZ results in enhanced AHR activation, suggesting that FICZ accumulates in the cell when its metabolism is blocked.^[1] FICZ significantly upregulates the gene expression of filaggrin in both HaCaT cells and NHEKs in an AHR-dependent manner, but does not affect the gene expression of other barrier-related proteins.^[2]
in vivo	FICZ improves the atopic dermatitis-like skin inflammation, clinical scores, and transepidermal water loss in NC/Nga mice compared with those of control mice. On histology, FICZ significantly reduces the epidermal and dermal thickness as well as the number of mast cells. Topical FICZ also significantly reduces the gene expression of Il22.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Human immortalized HaCaT cells, normal human epidermal keratinocytes (NHEKs)
	濃度	1 nM, 10 nM, 100 nM
	反応時間	1 h, 3 h, 5 h
	実験の流れ	HaCaTcells or NHEKs (1×10⁴ cells/well) are platedon an 8-well μ-slide for 48 h, and treated with or without FICZ for 3 or 1 h, respectively. Then, the cells are washed with phosphate-buffered saline (PBS), fixed with acetone for 10 min, and blocked with 5% (w/v) bovine serum albumin in PBS for 30 min. The cells are then incubated with primary rabbit anti-AHR antibody (1:100) overnight at 4 ℃. Specific binding is detected using a horseradish peroxidase-conjugated goat anti-rabbit anti-body followed by tyramide labeling with green-fluorescent Alexa Fluor 488 for 1 h at room temperature in accordance with the manufacturer's instructions.
動物実験	動物モデル	9–10 week-old female NC/Nga mice
	投薬量	100 mg/affected area
	投与方法	Topical

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Tubular aryl hydratocarbon receptor upregulates EZH2 to promote cellular senescence in cisplatin-induced acute kidney injury [ Cell Death Dis, 2023, 14(1):18]	PubMed: 36635272
Intestinal Flora-Derived Kynurenic Acid Protects Against Intestinal Damage Caused by Candida albicans Infection via Activation of Aryl Hydrocarbon Receptor [ Front Microbiol, 2022, 13:934786]	PubMed: 35923391
Novel Pentapeptide Derived from Chicken by-Product Ameliorates DSS-Induced Colitis by Enhancing Intestinal Barrier Function via AhR-Induced Src Inactivation [ J Agric Food Chem, 2020, 10.1021/acs.jafc.0c06319]	PubMed: 33210912

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。