FIN56

製品コードS8254 バッチS825401

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₅H₃₁N₃O₅S₂

分子量

517.66

CAS No.

1083162-61-1

Solubility (25°C)*

体外

DMSO

100 mg/mL (193.17 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	2%DMSO 1 40%PEG300 2 5%TWEEN80 3 53%dH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.280mg/ml (0.54mM)	Taking the 1 mL working solution as an example, add 20 μL of 14 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 530 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	FIN56は、フェロトーシスの特異的誘導剤です。
in vitro	FIN56 is a specific inducer of ferroptosis. The mechanism involves two distinct pathways: one pathway is degradation of GPX4, which requires the enzymatic activity of acetyl-CoA carboxylase; the other pathway is activation of squalene synthase, which leads to coenzyme Q10 depletion, in a manner independent of GPX4 degradation.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HT-1080 cells
	濃度	5 μM
	反応時間	10 h
	実験の流れ	500,000 HT-1080 cells are seeded in a 10-cm dish. Cells are grown at 37 °C for 16 h. On the day of the analysis, cells are cotreated with 100 μM α-tocopherol and either vehicle (DMSO) or a ferroptosis inducer (10 μM erastin, 0.5 μM (1S, 3R)-RSL3, or 5 μM FIN56) and incubated for 10 h. Cells are then trypsinized, pelleted, washed once with 400 μL of ice-cold PBS containing 1 mM EDTA, and sonicated. After the cell debris have been pelleted and removed, both oxidized and reduced glutathione in 120 μL of sample is quantified in technical triplicates. The glutathione quantity is normalized to the protein concentration measured via Bradford assay.

参考

https://pubmed.ncbi.nlm.nih.gov/27159577/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Cytosolic cytochrome c represses ferroptosis [ Cell Metab, 2025, S1550-4131(25)00149-4]	PubMed: 40233758
Ubiquinol-mediated suppression of mitochondria-associated ferroptosis is a targetable function of lactate dehydrogenase B in cancer [ Nat Commun, 2025, 16(1):2597]	PubMed: 40090955
Protection against ferroptosis through maintaining homeostasis of docosahexaenoate-containing phospholipids [ Mol Cell, 2025, 85(18):3474-3485.e5]	PubMed: 40934923
Activating the NFE2L1-ubiquitin-proteasome system by DDI2 protects from ferroptosis [ Cell Death Differ, 2025, 32(3):480-487]	PubMed: 39384955
GPX4 deficiency-induced ferroptosis drives endometrial epithelial fibrosis in polycystic ovary syndrome [ Redox Biol, 2025, 83:103615]	PubMed: 40253746
The deubiquitinase USP24 suppresses ferroptosis in triple-negative breast cancer by stabilizing DHODH protein [ Cell Death Dis, 2025, 16(1):564]	PubMed: 40715045
Ciprofloxacin enhances RSL3-induced ferroptosis by promoting mitochondrial Zn2+ accumulation via the STING1-CAV2 pathway [ J Biol Chem, 2025, 301(10):110653]	PubMed: 40889676
PKCiota Inhibits the Ferroptosis of Esophageal Cancer Cells via Suppressing USP14-Mediated Autophagic Degradation of GPX4 [ Antioxidants (Basel), 2024, 13(1)114]	PubMed: 38247539
The cell fate regulator DACH1 modulates ferroptosis through affecting P53/SLC25A37 signaling in fibrotic disease [ Hepatol Commun, 2024, 8(3)e0396]	PubMed: 38437058
OSGIN1 promotes ferroptosis resistance by directly enhancing GCLM activity [ Biochem Biophys Res Commun, 2024, 740:151015]	PubMed: 39571229

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。