FIN56

製品コードS8254 バッチS825401

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C25H31N3O5S2
分子量 517.66 CAS No. 1083162-61-1
Solubility (25°C)* 体外 DMSO 100 mg/mL (193.17 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 FIN56 is a specific inducer of ferroptosis .
in vitro FIN56 is a specific inducer of ferroptosis. The mechanism involves two distinct pathways: one pathway is degradation of GPX4, which requires the enzymatic activity of acetyl-CoA carboxylase; the other pathway is activation of squalene synthase, which leads to coenzyme Q10 depletion, in a manner independent of GPX4 degradation[1].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HT-1080 cells
濃度 5 μM
反応時間 10 h
実験の流れ

500,000 HT-1080 cells are seeded in a 10-cm dish. Cells are grown at 37 °C for 16 h. On the day of the analysis, cells are cotreated with 100 μM α-tocopherol and either vehicle (DMSO) or a ferroptosis inducer (10 μM erastin, 0.5 μM (1S, 3R)-RSL3, or 5 μM FIN56) and incubated for 10 h. Cells are then trypsinized, pelleted, washed once with 400 μL of ice-cold PBS containing 1 mM EDTA, and sonicated. After the cell debris have been pelleted and removed, both oxidized and reduced glutathione in 120 μL of sample is quantified in technical triplicates. The glutathione quantity is normalized to the protein concentration measured via Bradford assay.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

PKCiota Inhibits the Ferroptosis of Esophageal Cancer Cells via Suppressing USP14-Mediated Autophagic Degradation of GPX4 [ Antioxidants (Basel), 2024, 13(1)114] PubMed: 38247539
The cell fate regulator DACH1 modulates ferroptosis through affecting P53/SLC25A37 signaling in fibrotic disease [ Hepatol Commun, 2024, 8(3)e0396] PubMed: 38437058
Myeloid-like tumor hybrid cells in bone marrow promote progression of prostate cancer bone metastasis [ J Hematol Oncol, 2023, 16(1):46] PubMed: 37138326
Targeting NRF2 uncovered an intrinsic susceptibility of acute myeloid leukemia cells to ferroptosis [ Exp Hematol Oncol, 2023, 12(1):47] PubMed: 37198609
Selective ablation of primary and paracrine senescent cells by targeting iron dyshomeostasis [ Cell Rep, 2023, 42(2):112058] PubMed: 36753419
MMD collaborates with ACSL4 and MBOAT7 to promote polyunsaturated phosphatidylinositol remodeling and susceptibility to ferroptosis [ Cell Rep, 2023, 42(9):113023] PubMed: 37691145
Dihydroartemisinin-induced ferroptosis in acute myeloid leukemia: links to iron metabolism and metallothionein [ Cell Death Discov, 2023, 9(1):97] PubMed: 36928207
Ferroptosis heterogeneity in triple-negative breast cancer reveals an innovative immunotherapy combination strategy [ Cell Metab, 2022, S1550-4131-2200411-9] PubMed: 36257316
ACSL4-dependent ferroptosis does not represent a tumor-suppressive mechanism but ACSL4 rather promotes liver cancer progression [ Cell Death Dis, 2022, 13(8):704] PubMed: 35963845
Induction of autophagy-dependent ferroptosis to eliminate drug-tolerant human retinoblastoma cells [ Cell Death Dis, 2022, 13(6):521] PubMed: 35654783

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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