Folic acid

製品コードS4605 バッチS460501

化学情報

	Synonyms	Folacin, Vitamin B9, Vitamin M, Pteroylglutamic acid, Folate	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₉H₁₉N₇O₆
	分子量	441.40	CAS No.	59-30-3
Solubility (25°C)*	体外	DMSO	2 mg/mL warmed with 50ºC water bath (4.53 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Folic acid (Folacin, Vitamin B9, Vitamin M, Pteroylglutamic acid, Folate), a B vitamin, plays an important role in cell division and in the synthesis of amino acids and nucleic acids like DNA.
in vitro	Folic acid treatment induces dose-related increases in BRCA1 mRNA expression in HepG2, Huh-7D12, Hs578T, and JURKAT and in BRCA2 in HepG2, Hs578T, MCF7, and MDA-MB-157 cells. FA does not affect the corresponding normal cells or on any of the ovarian cell lines. Folic acid induces increased BRCA1 protein expression in Hs578T, but not HepG2 cells, whereas BRCA2 protein levels are undetectable. FA treatment does not alter DNA repair in liver-derived cells, whereas there are transient effects on breast-derived cells. There is no effect of FA treatment on BRCA1 or BRCA2 DNA methylation, although there is some variation in the methylation of specific CpG loci between some cell lines^[1].

製品説明

Folic acid (Folacin, Vitamin B9, Vitamin M, Pteroylglutamic acid, Folate), a B vitamin, plays an important role in cell division and in the synthesis of amino acids and nucleic acids like DNA.

in vitro

Folic acid treatment induces dose-related increases in BRCA1 mRNA expression in HepG2, Huh-7D12, Hs578T, and JURKAT and in BRCA2 in HepG2, Hs578T, MCF7, and MDA-MB-157 cells. FA does not affect the corresponding normal cells or on any of the ovarian cell lines. Folic acid induces increased BRCA1 protein expression in Hs578T, but not HepG2 cells, whereas BRCA2 protein levels are undetectable. FA treatment does not alter DNA repair in liver-derived cells, whereas there are transient effects on breast-derived cells. There is no effect of FA treatment on BRCA1 or BRCA2 DNA methylation, although there is some variation in the methylation of specific CpG loci between some cell lines^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HepG2, Huh-7D12, Hs578T, JURKAT, Hs578T, MCF7, and MDA-MB-157 cells
	濃度	0, 25, 50, 75, or 100 nmol/L
	反応時間	72 h
	実験の流れ	To determine the effect of FA supplementation on BRCA1 and BRCA2 mRNA expression, all cell lines were treated with 0, 25, 50, 75, or 100 nmol/L FA for 72 hours before harvesting in TRI Reagent according to the manufacturer’s instructions.
動物実験	動物モデル	ICR strain mice
	投薬量	3 mg/kg
	投与方法	by gastric intubation

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

HDAC3 aberration-incurred GPX4 suppression drives renal ferroptosis and AKI-CKD progression [ Redox Biol, 2023, 10.1016/j.redox.2023.102939]	PubMed: 37890360
Hyperhomocysteinemia Promotes Cardiac Hypertrophy in Hypertension [ Oxid Med Cell Longev, 2022, 2022:1486157]	PubMed: 36046692
Repurposing the natural compounds as potential therapeutic agents for COVID-19 based on the molecular docking study of the main protease and the receptor-binding domain of spike protein [ J Mol Model, 2022, 28(6):153]	PubMed: 35578055

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。