Forskolin (Colforsin)

製品コードS2449 バッチS244906

印刷

化学情報

Chemical Structure Synonyms HL 362, Coleonol Storage
(From the date of receipt)		 3 years -20°C(in the dark) powder
1 year -80°C(in the dark) in solvent
化学式

C22H34O7
分子量 410.5 CAS No. 66575-29-9
Solubility (25°C)* 体外 DMSO 82 mg/mL (199.75 mM)
Ethanol 45 mg/mL (109.62 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 コルホルチン (Forskolin (Colforsin)) は、広範な細胞腫における真核生物のアデニル酸シクラーゼ (adenylate cyclase, AC) の遍在する活性化因子であり、細胞生理学の研究において cAMP のレベルを上げるために一般的に使用されています。ホルスコリン (Forskolin) は、PXR と FXR も活性化します。 ホルスコリンはオートファジー (autophagy) を刺激します。
in vitro

Forskolin increases cAMP levels in preparations of membranes, cells, or tissues. Forskolin not only activates AC but also interacts with certain other proteins, including glucose transporters and ion channels. Forskolin is able to promote activation of nine different transmembrane iso-forms of AC, albeit with somewhat less efficacy for AC9, which could be used to provides a means to identify and quantify high-affinity binding sites, i.e., G-proteins (Gs)–AC complexes. Activation of s by GPCRs contributes to Forskolin-stimulated cAMP generation in cells because of s-Forskolin potentiation of AC activity. [1] Forskolin stimulates adenylate cyclase activity without interacting with cell surface receptors. Forskolin's potentiation of cAMP in turn inhibits basophil and mast cell degranulation and histamine release, lowers blood pressure and intraocular pressure, inhibits platelet aggregation, promotes vasodilation, bronchodilation, and thyroid hormone secretion, and stimulates lipolysis in fat cells. Forskolin inhibits the binding of platelet-activating factor (PAF), independently of cAMP formation, which may be a result of Forskolin's direct effect on PAF or via interference with PAF binding to receptor sites. Forskolin also appears to have an effect on several membrane transport proteins, and inhibits glucose transport in erythrocytes, adipocytes, platelets, and other cells. Forskolin is used to treat with glaucoma. [2]
in vivo

Colforsin (Forskolin, HL 362, Coleonol) is a ubiquitous activator of eukaryotic adenylyl cyclase (AC) in a wide variety of cell types, commonly used to raise levels of cAMP. It also activates PXR and FXR activity and stimulates autophagy.[4]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 hMPCs or cynMPCs
濃度 20 μM
反応時間 2 h
実験の流れ

Cells were treated with 20 μM forskolin (S2449, Selleck) for 2 h for synchronization.
動物実験 動物モデル Male Sprague-Dawley rats
投薬量 10 mg/kg
投与方法 i.p.

カスタマーフィードバック

Data from [Eur J Cancer, 2014, 50(7), 1310-20]

Data from [Data independently produced by , , Clin Cancer Res, 2018, doi:10.1158/1078-0432.CCR-18-2912]

Data from [Data independently produced by , , J Exp Clin Cancer Res, 2019, 37(1):288]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Preclinical efficacy and safety of encapsulated proliferating human hepatocyte organoids in treating liver failure [ Cell Stem Cell, 2024, S1934-5909(24)00048-1] PubMed: 38458193
Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9 [ iScience, 2024, 27(2):108857] PubMed: 38303710
IL-6-Derived Autocrine Lactate Promotes Immune Escape of Uveal Melanoma [ Invest Ophthalmol Vis Sci, 2024, 65(3):37] PubMed: 38551584
Sox9 links biliary maturation to branching morphogenesis [ bioRxiv, 2024, 2024.01.15.574730] PubMed: 38293117
A stem cell zoo uncovers intracellular scaling of developmental tempo across mammals [ Cell Stem Cell, 2023, 30(7):938-949.e7] PubMed: 37343565
A placental model of SARS-CoV-2 infection reveals ACE2-dependent susceptibility and differentiation impairment in syncytiotrophoblasts [ Nat Cell Biol, 2023, 25(8):1223-1234] PubMed: 37443288
Kinase-Modulated Bioluminescent Indicators Enable Noninvasive Imaging of Drug Activity in the Brain [ ACS Cent Sci, 2023, 9(4):719-732] PubMed: 37122464
Rescue of alveolar wall liquid secretion blocks fatal lung injury due to influenza-staphylococcal coinfection [ J Clin Invest, 2023, 10.1172/JCI163402] PubMed: 37581936
Rescue of alveolar wall liquid secretion blocks fatal lung injury due to influenza-staphylococcal coinfection [ J Clin Invest, 2023, 133(19)e163402] PubMed: 37581936
Metabolic classification suggests the GLUT1/ALDOB/G6PD axis as a therapeutic target in chemotherapy-resistant pancreatic cancer [ Cell Rep Med, 2023, 4(9):101162] PubMed: 37597521

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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