Datasheet

生物学的記述

Specificity	GDF-15 Antibody [G11L21] detects endogenous levels of total GDF-15 protein.
Background	GDF-15 (Growth/Differentiation Factor 15, also known as MIC-1) is a stress-inducible cytokine and divergent member of the TGF-β superfamily, synthesized as an inactive precursor that is proteolytically cleaved at a furin-like RXXR site to produce a mature, disulfide-linked dimer via a conserved cysteine knot motif. GDF-15 is secreted in its mature form and signals predominantly through the GFRAL receptor in the hindbrain, activating MAPK/ERK and SMAD pathways independently of canonical TGF-β receptors. GDF-15 is markedly upregulated in response to cellular stress, inflammation, mitochondrial dysfunction, and tissue injury, serving as a sensitive biomarker for perioperative risk, organ damage, and postoperative complications, often outperforming traditional markers like CRP or BNP. It modulates immune responses by restraining macrophage activity, protects tissue integrity through ERK/Akt signaling and mitochondrial stabilization, and exerts metabolic effects such as appetite suppression and weight loss relevant to bariatric surgery. Elevated GDF-15 indicates immune dysfunction in sepsis and contributes to cancer cachexia through p53-dependent transcriptional mechanisms.

Specificity

GDF-15 Antibody [G11L21] detects endogenous levels of total GDF-15 protein.

Background

GDF-15 (Growth/Differentiation Factor 15, also known as MIC-1) is a stress-inducible cytokine and divergent member of the TGF-β superfamily, synthesized as an inactive precursor that is proteolytically cleaved at a furin-like RXXR site to produce a mature, disulfide-linked dimer via a conserved cysteine knot motif. GDF-15 is secreted in its mature form and signals predominantly through the GFRAL receptor in the hindbrain, activating MAPK/ERK and SMAD pathways independently of canonical TGF-β receptors. GDF-15 is markedly upregulated in response to cellular stress, inflammation, mitochondrial dysfunction, and tissue injury, serving as a sensitive biomarker for perioperative risk, organ damage, and postoperative complications, often outperforming traditional markers like CRP or BNP. It modulates immune responses by restraining macrophage activity, protects tissue integrity through ERK/Akt signaling and mitochondrial stabilization, and exerts metabolic effects such as appetite suppression and weight loss relevant to bariatric surgery. Elevated GDF-15 indicates immune dysfunction in sepsis and contributes to cancer cachexia through p53-dependent transcriptional mechanisms.

使用情報

Application

WB, IP, IF, ELISA

Dilution

WB	IF
1:100-1:1000	1:50-1:500

Reactivity

Human, Mouse, Rat

Source

Mouse Monoclonal Antibody

MW

40 kDa (GDF-15 precursor), 30 kDa (mature GDF-15)

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/32508832/
https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2018.01712/full

Application Data

WB

Validated by Selleck

Lane 1: LNCap, Lane 2: C6, Lane 3: RAW264.7