受注:045-509-1970 |
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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C20H40N4O10.2H2SO4 |
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分子量 | 692.71 | CAS No. | 108321-42-2 | |
Solubility (25°C)* | 体外 | Water | 100 mg/mL warmed with 50ºC water bath (144.36 mM) | |
DMSO | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Geneticin (G418 Sulfate), an aminoglycoside antibiotic, is an elongation inhibitor of 80 S ribosomes that blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. |
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in vitro | G418 is an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/ml. Resistance to G418 conferring by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APT 3' II is commonly used in laboratory research to select genetically engineered cells. [1] In general for bacteria and algae concentrations of 5 mg/L or less are used, for mammalian cells concentrations of approximately 400 mg/L are used for selection and 200 mg/L for maintenance. Resistant clones selection could require from 1 to up to 3 weeks. [2] |
in vivo | Doses of G418 of between 40 and 80 mg/kg for three consecutive days are sufficient to eliminate all nontransfected T. brucei brucei parasites from infected mice. [3] |
細胞アッセイ | 細胞株 | A549 cells |
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濃度 | 494.1 μg/ml | |
反応時間 | ||
実験の流れ | Cells were treated with different concentrations (100-1200 μg/ml) of genticin and MTT assay was performed to identify IC50. |
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動物実験 | 動物モデル | Sublethally irradiated mice with bloodstream forms of T. bruceibrucei GUTat 3.1 and T. brucei brucei GUTat 3.1/BBR3. |
投薬量 | 10, 20, 30, 40, 50, or 80 mg/kg | |
投与方法 | I.P. |
USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473] | PubMed: 37833415 |
USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473] | PubMed: 37833415 |
TSPAN18 facilitates bone metastasis of prostate cancer by protecting STIM1 from TRIM32-mediated ubiquitination [ J Exp Clin Cancer Res, 2023, 42(1):195] | PubMed: 37542345 |
LMO2 promotes the development of AML through interaction with transcription co-regulator LDB1 [ Cell Death Dis, 2023, 14(8):518] | PubMed: 37573405 |
LMO2 promotes the development of AML through interaction with transcription co-regulator LDB1 [ Cell Death Dis, 2023, 14(8):518] | PubMed: 37573405 |
HIF2α-induced upregulation of RNASET2 promotes triglyceride synthesis and enhances cell migration in clear cell renal cell carcinoma [ FEBS Open Bio, 2023, 13(4):638-654] | PubMed: 36728187 |
HIF2α-induced upregulation of RNASET2 promotes triglyceride synthesis and enhances cell migration in clear cell renal cell carcinoma [ FEBS Open Bio, 2023, none] | PubMed: 36728187 |
A multimodal iPSC platform for cystic fibrosis drug testing [ Nat Commun, 2022, 13(1):4270] | PubMed: 35906215 |
Targeting glutamine metabolism in hepatic stellate cells alleviates liver fibrosis [ Cell Death Dis, 2022, 13(11):955] | PubMed: 36376267 |
Synthetic lethality of cyclin-dependent kinase inhibitor Dinaciclib with VHL-deficiency allows for selective targeting of clear cell renal cell carcinoma [ Cell Cycle, 2022, 1-17] | PubMed: 35240916 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。