Geneticin (G418 Sulfate)

製品コードS3028 バッチS302811

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₂₀H₄₀N₄O₁₀.2H₂SO₄
	分子量	692.71	CAS No.	108321-42-2
Solubility (25°C)*	体外	Water	100 mg/mL warmed with 50ºC water bath (144.36 mM)
		DMSO	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Geneticin (G418 Sulfate), an aminoglycoside antibiotic, is an elongation inhibitor of 80 S ribosomes that blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.
in vitro	G418 is an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/ml. Resistance to G418 conferring by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APT 3' II is commonly used in laboratory research to select genetically engineered cells. ^[1] In general for bacteria and algae concentrations of 5 mg/L or less are used, for mammalian cells concentrations of approximately 400 mg/L are used for selection and 200 mg/L for maintenance. Resistant clones selection could require from 1 to up to 3 weeks. ^[2]
in vivo	Doses of G418 of between 40 and 80 mg/kg for three consecutive days are sufficient to eliminate all nontransfected T. brucei brucei parasites from infected mice. ^[3]

製品説明

Geneticin (G418 Sulfate), an aminoglycoside antibiotic, is an elongation inhibitor of 80 S ribosomes that blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.

in vitro

G418 is an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/ml. Resistance to G418 conferring by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APT 3' II is commonly used in laboratory research to select genetically engineered cells. ^[1]

In general for bacteria and algae concentrations of 5 mg/L or less are used, for mammalian cells concentrations of approximately 400 mg/L are used for selection and 200 mg/L for maintenance. Resistant clones selection could require from 1 to up to 3 weeks. ^[2]

in vivo

Doses of G418 of between 40 and 80 mg/kg for three consecutive days are sufficient to eliminate all nontransfected T. brucei brucei parasites from infected mice. ^[3]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	A549 cells
	濃度	494.1 μg/ml
	反応時間
	実験の流れ	Cells were treated with different concentrations (100-1200 μg/ml) of genticin and MTT assay was performed to identify IC50.
動物実験	動物モデル	Sublethally irradiated mice with bloodstream forms of T. bruceibrucei GUTat 3.1 and T. brucei brucei GUTat 3.1/BBR3.
	投薬量	10, 20, 30, 40, 50, or 80 mg/kg
	投与方法	I.P.

参考

http://www.ncbi.nlm.nih.gov/pubmed?term=7001938
http://www.ncbi.nlm.nih.gov/pubmed?term=14562372
http://www.ncbi.nlm.nih.gov/pubmed?term=8517708

https://pubmed.ncbi.nlm.nih.gov/30594785/
https://pubmed.ncbi.nlm.nih.gov/20729841/

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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