Gentisic acid

製品コードS3799 バッチS379901

印刷

化学情報

Chemical Structure Synonyms DHB, 2,5-dihydroxybenzoic acid, 5-Hydroxysalicylic acid, Gentianic acid, Carboxyhydroquinone, Hydroquinonecarboxylic acid Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C7H6O4
分子量 154.12 CAS No. 490-79-9
Solubility (25°C)* 体外 DMSO 30 mg/mL (194.65 mM)
Water 7.5 mg/mL (48.66 mM)
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Gentisic acid (2,5-dihydroxybenzoic acid, 5-Hydroxysalicylic acid, Hydroquinonecarboxylic acid, DHB), an active metabolite of salicylic acid degradation, has a broad spectrum of biological activity, such as anti-inflammatory, antirheumatic and antioxidant properties.
in vitro

Gentisic acid (GA) has been shown to act as an antioxidant under different types of physical and chemical stimulus. Gentisic acid efficiently protects lipids, proteins, SOD enzyme and human erythrocytes on gamma radiation exposure. It has been found to scavenge physiologically relevant hydroxyl and peroxyl radicals in pulse radiolysis studies[3].
in vivo

Gentisic acid (GA) shows a potent protective effect against CP induced hepatotoxicity and genotoxicity. The protective effect of Gentisic acid can be attributed to its antioxidant properties, which enhance natural antioxidant enzymes and the cell membrane stabilizing property by inhibiting lipid peroxidation[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HTC cells
濃度 0.08, 0.16, 0.8, 1.6, and 8 μg/mL
反応時間 24 h
実験の流れ

The Trypan Blue exclusion protocol is assessed in 24-well cell culture plates. In each well, 2.5×cells are seeded in 2 mL of complete culture medium with the following treatments: control (CO, 50 μL of culture medium, as negative control), cytotoxic agent doxorubicin (DXR, 10 μg/mL, as positive control), and Gentisic acid (0.08, 0.16, 0.8, 1.6, and 8 μg/mL). The cells are incubated for 24 h, trypsinized and then resuspended in 0.5 mL of culture medium. An aliquot of the cellular suspension (20 μL) is homogenized with Trypan Blue (0.2%) dye in a 1:1 ratio. The cells are quantified with a Neubauer chamber hemocytometer.
動物実験 動物モデル male Swiss albino mice
投薬量 50 and 100 mg/kg
投与方法 oral administration

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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