Glucosamine sulfate

製品コードS3850 バッチS385001

印刷

化学情報

Chemical Structure Synonyms D-Glucosaminesulfate, D-Glucosamine sulphate Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C6H13NO5.H2SO4
分子量 277.25 CAS No. 29031-19-4
Solubility (25°C)* 体外 Water 55 mg/mL (198.37 mM)
DMSO 4 mg/mL (14.42 mM)
 
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Glucosamine sulfate (D-Glucosaminesulfate, D-Glucosamine sulphate) is a dietary supplement commonly used in the treatment of osteoarthritis. It is a type of glycosaminoglycan, which is a naturally occurring major component of joint cartilage and joint fluid.
in vitro Glucosamine sulfate exerts a pronounced suppressive effect on MMPs in osteosarcoma cell lines in vitro[1]. Glucosamine suppresses the activation of T-lymphoblasts and dendritic cells in vitro as well as allogeneic mixed leukocyte reactivity in a dose-dependent manner. Glucosamine has been shown to inhibit nucleic acid and protein biosynthesis and irreversible damage to organelles in tumor cells, but not in normal cells[3].
in vivo Glucosamine sulfate attenuates DSS-induced colitis symptoms, possibly by suppressing the NF-κB-mediated TNF-α and IL-1β production and neutrophil activation that contribute to intestinal barrier disruption in colitic mice[2]. Glucosamine administration prolongs allogeneic cardiac allograft survival in vivo[3].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 MG-63 and SaOS-2 cells
濃度 0, 10, 50 and 100 μg/ml
反応時間 48 h
実験の流れ Cells are exposed to 0, 10, 50 and 100 μg/ml glucosamine sulfate for 48 h and treatment toxicity is determined through measurement of cell viability and proliferation. Relative gene expression of matrix metalloproteinase (MMP)-2, -3 and -9 is quantified by real-time polymerase chain reaction. Protein levels of MMP-2 and -9 are assessed by ELISA.
動物実験 動物モデル C57BL/6 mice
投薬量 0, 0.05, and 0.10% (w/w)
投与方法 oral

参考

  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000453/
  • https://pubmed.ncbi.nlm.nih.gov/24325781/
  • http://www.jbc.org/content/277/42/39343.full?utm_source=TrendMD&utm_medium=cpc&utm_campaign=Journal_of_Biological_Chemistry_TrendMD_0

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。