Glycitin

製品コードS3825 バッチS382501

印刷

化学情報

Synonyms

Glycitein-7-β-O-glucoside

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₂H₂₂O₁₀

分子量

446.40

CAS No.

40246-10-4

Solubility (25°C)*

体外

DMSO

89 mg/mL (199.37 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Glycitin (Glycitein-7-β-O-glucoside), a natural isoflavone isolated from legumes, has antibacterial, antiviral and estrogenic activities and may exerts preventative effects on alcoholism, cardiovascular and cerebrovascular diseases and some types of cancer.
in vitro	Glycitin promotes cell proliferation, osteoblast induction, and activates Col I mRNA expression and ALP activity of BMSCs. This compound promotes the proliferation and migration of human dermal fibroblast cells through TGF-β and p-AKT signaling^[1].
in vivo	Glycitin significantly prevents bone loss in variectomized (ovx) rats at a dose of 50 mg/kg/d. At this dose this compound also prevents ovx-induced uterine atrophy and increases in body weight gain, abdominal fat, serum total cholesterol and triglyceride, and urinary excretion of pyridinoline and deoxypyridinoline with statistical significance^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Bone marrow stem cells (BMSCs)
	濃度	0.01, 0.5, 1, 5 and 10 µM
	反応時間	7 days
	実験の流れ	BMSCs (1-2×10⁶ cells or 1-2×10⁴ per well) are cultured in 6- or 96-well culture plates overnight at 37°C in an atmosphere containing 5% CO₂. Glycitin is added to the wells at final concentrations of 0.01, 0.5, 1, 5 and 10 µM and cultured for 7 days. In cells cultured in 6-well culture plates, BMSCs are determined using Oil Red O staining and observed via light microscopy at 510 nm. BMSCs are fixed using 5% precooled paraformaldehyde for 30 min at 4°C and stained with 0.6% (w/v) Oil Red O solution for 15 min at room temperature. Cells stained with Oil Red O are washed with water (3×5 min) to remove unbound dye, and culture dishes are stained with 1 ml isopropyl alcohol for 10 min. In cells cultured in 96-well culture plates, BMSCs are determined via MTT assay. A total of 20 µl MTT (5 g/l) are added to each well and cultured for 4 h. The supernatant is removed and 200 µl dimethylsulfoxide are added to each well for 15 min. Optical density (OD) is measured using a microplate spectrophotometer at 570 nm. Proliferation rate is calculated using: OD treated / OD control × 100%.
動物実験	動物モデル	Female Sprague-Dawley rats
	投薬量	25, 50 or 100 mg/kg/d
	投与方法	oral

参考

https://pubmed.ncbi.nlm.nih.gov/27882117/
https://pubmed.ncbi.nlm.nih.gov/11305597/

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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