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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C11H14ClN3O5 |
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分子量 | 303.7 | CAS No. | 67953-08-6 | |
Solubility (25°C)* | 体外 | DMSO | 5 mg/mL (16.46 mM) | |
Water | 1 mg/mL warmed with 50ºC water bath (3.29 mM) | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | GPNA (L-γ-Glutamyl-p-nitroanilide) is a potent and selective inhibitor of the glutamine (Gln) transporter ASCT2(SLC1A5) with Ki of 55 µM. |
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in vitro | GPNA leads to a significant decrease in endometrial cancer cell growth and spheroid cross-sectional area.[1] |
in vivo | EGFRAS-GPNA (EGFRAS-specific GPNA oligomers) is taken-up by several tissues including the xenograft tumor. Systemic administration of EGFRAS-GPNA induces antitumor effects in HNSCC xenografts, with similar efficacies as the FDA-approved EGFR inhibitors: cetuximab and erlotinib. EGFRAS-GPNA effectively againsts the constitutively active EGFR vIII mutant implicated in cetuximab resistance. |
細胞アッセイ | 細胞株 | Endometrial cancer cell line |
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濃度 | 1 mM | |
反応時間 | 24 h | |
実験の流れ | Cells (1–2 × 105) are cultured for 24 h followed by treatment where stated with vehicle or 1 mm GPNA for 24 h. And then staining for flow cytometric analysis is performed. MTT assays are conducted using 100–250 cells per well in a 96-well plate. Fresh media with vehicle or 1 mm GPNA are applied every two days. |
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動物実験 | 動物モデル | HNSCC xenograft-bearing nude mice |
投薬量 | 5 mg/kg | |
投与方法 | i.p. |
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Lipophagy-mediated cholesterol synthesis inhibition is required for the survival of hepatocellular carcinoma under glutamine deprivation [ Redox Biol, 2023, 63:102732] | PubMed: 37150151 |
Inhibition of SND1 overcomes chemoresistance in bladder cancer cells by promoting ferroptosis [ Oncol Rep, 2023, 49(1)16] | PubMed: 36453257 |
Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3SPOP E3 ligase in cancer cells [ Nat Commun, 2022, 13(1):3034] | PubMed: 35641493 |
Antagonizing Glutamine Bioavailability Promotes Radiation Sensitivity in Prostate Cancer [ Cancers (Basel), 2022, 14(10)2491] | PubMed: 35626095 |
Evaluation of Glutaminolysis in T Cells [ Curr Protoc, 2022, 2(9):e540] | PubMed: 36111948 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。