Guanidine HCl

製品コードS4070 バッチS407001

印刷

化学情報

Synonyms

Aminoformamidine HCl

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

CH₅N₃.HCl

分子量

95.53

CAS No.

50-01-1

Solubility (25°C)*

体外

DMSO

19 mg/mL (198.89 mM)

Water

19 mg/mL (198.89 mM)

Ethanol

6 mg/mL (62.8 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Guanidine HCl (Aminoformamidine), the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant.
in vitro	Guanidine HCl is the most popular protein denaturant. Analysis of unfolding transitions by this compound provides several important parameters regarding the mechanism of conformational stability of proteins. ^[1] It at low concentrations refolds acid-unfolds apomyoglobin and cytochrome c, stabilizing the molten globule state. This chemical (> 1 M) causes co-operative unfolding of the molten globule state. ^[2] It is 2.8 times more effective than urea in unfolding ribonuclease but only 1.7 times more effective for lysozyme. △G^H₂O_app. values of this compound are 9.7 Cal/mole for ribonuclease at pH 6.6, 6.1 for lysozyme at pH 2.9, 8.3 for α-chymotrypsin at pH 4.3, and 11.7 for β-lactoglobulin at pH 3.2. ^[3] It at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI⁺] element from yeast cells. 5 mM of this chemical in growth media cures [PSI⁺] and other prions of yeast. ^[4] 5 mM of this compound significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. It also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase. ^[5]

製品説明

Guanidine HCl (Aminoformamidine), the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant.

in vitro

Guanidine HCl is the most popular protein denaturant. Analysis of unfolding transitions by this compound provides several important parameters regarding the mechanism of conformational stability of proteins. ^[1] It at low concentrations refolds acid-unfolds apomyoglobin and cytochrome c, stabilizing the molten globule state. This chemical (> 1 M) causes co-operative unfolding of the molten globule state. ^[2] It is 2.8 times more effective than urea in unfolding ribonuclease but only 1.7 times more effective for lysozyme. △G^H₂O_app. values of this compound are 9.7 Cal/mole for ribonuclease at pH 6.6, 6.1 for lysozyme at pH 2.9, 8.3 for α-chymotrypsin at pH 4.3, and 11.7 for β-lactoglobulin at pH 3.2. ^[3] It at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI⁺] element from yeast cells. 5 mM of this chemical in growth media cures [PSI⁺] and other prions of yeast. ^[4] 5 mM of this compound significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. It also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase. ^[5]

プロトコル(参考用のみ)

参考

https://pubmed.ncbi.nlm.nih.gov/3773761/
https://pubmed.ncbi.nlm.nih.gov/8389881/
https://pubmed.ncbi.nlm.nih.gov/4416801/

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC26647/
https://pubmed.ncbi.nlm.nih.gov/11375656/

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Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Cevipabulin-tubulin complex reveals a novel agent binding site on α-tubulin with tubulin degradation effect [ Sci Adv, 2021, 7(21)eabg4168]	PubMed: 34138737

Cevipabulin-tubulin complex reveals a novel agent binding site on α-tubulin with tubulin degradation effect [ Sci Adv, 2021, 7(21)eabg4168]

PubMed: 34138737

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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