Hemin

製品コードS5645 バッチS564501

印刷

化学情報

Synonyms

protohemin

Storage
(From the date of receipt)

3 years -20°C(in the dark) powder

化学式

C₃₄H₃₂ClFeN₄O₄

分子量

651.94

CAS No.

16009-13-5

Solubility (25°C)*

体外

DMSO

20 mg/mL (30.67 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

5%DMSO 1 Corn oil

1.0mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Hemin (protohemin) is an iron-containing porphyrin used in the management of porphyria attacks, particularly in acute intermittent porphyria.This product has poor solubility, animal experiments are available, cell experiments please choose carefully!
in vitro	Treatment of hemin or AFC damped the expression of TFRC in CT26 cells, which can be significantly rescued by overexpression of OTUD1.^[1]
in vivo	Following the i.p. administration of Hemin (100 μM/kg), the HO-1 level in the renal cortex begins to increase gradually. The HO-1 level reaches its peak 24 h after Hemin preconditioning. HO-1 is expressed mainly in the renal tubules. The HO-2 level in the kidney does not change following Hemin preconditioning.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	CT26 cells
	濃度	100 μM
	反応時間	30 min
	実験の流れ	Mock‐ and OTUD1‐expressing CT26 cells are treated with hemin (100 μM). Flow cytometric analyses the TFRC expression.
動物実験	動物モデル	mouse model of renal ischemia-reperfusion (8-10 week old male BABL/c mice)
	投薬量	100 μM/kg
	投与方法	i.p.

参考

[1] Song J, et al. EMBO Rep. 2021 Feb 3;22(2):e51162.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Saracatinib prompts hemin-induced K562 erythroid differentiation but suppresses erythropoiesis of hematopoietic stem cells [ Hum Cell, 2024, 10.1007/s13577-024-01034-5]	PubMed: 38388899
Tissue-resident Lachnospiraceae family bacteria protect against colorectal carcinogenesis by promoting tumor immune surveillance [ Cell Host Microbe, 2023, 31(3):418-432.e8]	PubMed: 36893736
Indoleamine 2, 3-Dioxygenase 1 Mediates Survival Signals in Chronic Lymphocytic Leukemia via Kynurenine/Aryl Hydrocarbon Receptor-Mediated MCL1 Modulation [ Front Immunol, 2022, 13:799331]	PubMed: 35296076
Heme induces intestinal epithelial cell ferroptosis via mitochondrial dysfunction in transfusion-associated necrotizing enterocolitis [ FASEB J, 2022, 36(12):e22649]	PubMed: 36383399
The deubiquitinase OTUD1 enhances iron transport and potentiates host antitumor immunity [ EMBO Rep, 2021, 22(2):e51162]	PubMed: 33393230
pH-dependent rearrangement determines the iron-activation and antitumor activity of artemisinins [ Free Radic Biol Med, 2021, 163:234-242]	PubMed: 33359684

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。