Datasheet

生物学的記述

Specificity	hnRNP A2B1 Antibody [A10M11] detects endogenous levels of total hnRNP A2B1 protein.
Background	hnRNP A2B1, a member of the heterogeneous nuclear ribonucleoprotein family, functions as a versatile RNA-binding protein that shuttles between nucleus and cytoplasm to orchestrate pre-mRNA processing and mRNA transport. It consists of two N-terminal RNA recognition motifs (RRMs) for sequence-specific binding to A2 response elements (A2REs) on target transcripts and a glycine-rich C-terminal domain that mediates protein-protein interactions and phase separation into membraneless organelles. In RNA metabolism, hnRNP A2B1 binds nascent pre-mRNAs to regulate alternative splicing of cell proliferation genes like RON and CD44 by competing with U2AF65 at polypyrimidine tracts, while also packaging mature mRNAs into RNP granules for nuclear export and dendritic localization in neurons. The protein activates STAT3 and ERK1/2 signaling through splicing of oncogenic isoforms that encode cytoplasmic variants with enhanced signaling potential, promoting epithelial-mesenchymal transition and invasion in breast cancer cells. In tauopathies, hnRNP A2B1 forms a pathological complex with oligomeric tau and N6-methyladenosine (m6A)-marked RNAs, sequestering transcripts into stress granules that impair global translation and trigger integrated stress response via eIF2α phosphorylation. hnRNP A2B1 directs transport of β-actin mRNA to neuronal growth cones via A2RE recognition, supporting synapse formation and plasticity. Knockdown disrupts m6A reader function, releases tau-induced translation block, and ameliorates neurodegeneration in P301S tau mice. Overexpression correlates with poor prognosis in breast cancer subtypes, while cytoplasmic mislocalization appears in Alzheimer's disease brains alongside tau aggregates.

Specificity

hnRNP A2B1 Antibody [A10M11] detects endogenous levels of total hnRNP A2B1 protein.

Background

hnRNP A2B1, a member of the heterogeneous nuclear ribonucleoprotein family, functions as a versatile RNA-binding protein that shuttles between nucleus and cytoplasm to orchestrate pre-mRNA processing and mRNA transport. It consists of two N-terminal RNA recognition motifs (RRMs) for sequence-specific binding to A2 response elements (A2REs) on target transcripts and a glycine-rich C-terminal domain that mediates protein-protein interactions and phase separation into membraneless organelles. In RNA metabolism, hnRNP A2B1 binds nascent pre-mRNAs to regulate alternative splicing of cell proliferation genes like RON and CD44 by competing with U2AF65 at polypyrimidine tracts, while also packaging mature mRNAs into RNP granules for nuclear export and dendritic localization in neurons. The protein activates STAT3 and ERK1/2 signaling through splicing of oncogenic isoforms that encode cytoplasmic variants with enhanced signaling potential, promoting epithelial-mesenchymal transition and invasion in breast cancer cells. In tauopathies, hnRNP A2B1 forms a pathological complex with oligomeric tau and N6-methyladenosine (m6A)-marked RNAs, sequestering transcripts into stress granules that impair global translation and trigger integrated stress response via eIF2α phosphorylation. hnRNP A2B1 directs transport of β-actin mRNA to neuronal growth cones via A2RE recognition, supporting synapse formation and plasticity. Knockdown disrupts m6A reader function, releases tau-induced translation block, and ameliorates neurodegeneration in P301S tau mice. Overexpression correlates with poor prognosis in breast cancer subtypes, while cytoplasmic mislocalization appears in Alzheimer's disease brains alongside tau aggregates.

使用情報

Application

WB, IHC, IF, FCM

Dilution

WB	IHC	IF	FCM
1:1000	1:5000	1:1000	1:500

Reactivity

Rat, Mouse, Human

Source

Rabbit Monoclonal Antibody

MW

37 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/28351333/
https://pubmed.ncbi.nlm.nih.gov/32588964/

hnRNP A2B1 Antibody [A10M11]

生物学的記述

使用情報

References

Application Data