HO-3867

製品コードS7501 バッチS750101

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₈H₃₀F₂N₂O₂

分子量

464.55

CAS No.

1172133-28-6

Solubility (25°C)*

体外

DMSO (warmed with 50ºC water bath)

13 mg/mL (27.98 mM)

Ethanol (warmed with 50ºC water bath)

6 mg/mL (12.91 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.650mg/ml (1.40mM)	Taking the 1 mL working solution as an example, add 50 μL of 13 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.330mg/ml (0.71mM)	Taking the 1 mL working solution as an example, add 50 μL of 6.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	HO-3867, an analog of curcumin, is a selective STAT3 inhibitor that inhibits its phosphorylation, transcription, and DNA binding without affecting the expression of other active STATs. HO-3867 induces apoptosis.
in vitro	HO-3867 produces significant cytotoxicity in A2780 and other tested ovarian cancer cell lines, with less toxic to noncancerous ovarian surface epithelial cells. This compound induces G(2)-M cell cycle arrest in A2780 cells and promotes apoptosis by caspase-8 and caspase-3 activation. It blocks the JAK/STAT3 pathway in human ovarian cancer cell lines. ^[1]
in vivo	HO-3867 (100 ppm p.o.) inhibits the growth of ovarian cancer xenograft tumor in mice without any apparent signs of toxicity, and also results in inhibition of pSTAT3 as well as downregulation of the STAT3-targeting proteins. ^[1] This compound sensitizes cisplatin-resistant ovarian carcinoma through STAT3 inhibition. ^[2] It also attenuates left-heart-failure-induced pulmonary hypertension by decreasing oxidative stress and increasing PTEN expression in the lung of rats. ^[3]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	A2780 (A2780R), PA-1, SKOV3, OV4, and OVCAR3 cells; human ovarian surface epithelial cell
	濃度	~20 μM
	反応時間	24 hours
	実験の流れ	Cell viability is determined by a colorimetric assay using MTT. In the mitochondria of living cells, yellow MTT undergoes a reductive conversion to formazan, producing a purple color. Cells, grown to ~80% confluence in 75-mm flasks, are trypsinized, counted, seeded in 96-well plates with an average population of 7,000 cells/well, incubated overnight, and then treated with HO-3867 for 24 h. All experiments are done using 8 replicates and repeated at least three times.
動物実験	動物モデル	Mice bearing ovarian cancer A2780 tumor xenografts
	投薬量	~100 ppm
	投与方法	p.o.

参考

https://pubmed.ncbi.nlm.nih.gov/2044/
https://pubmed.ncbi.nlm.nih.gov/21885917/
https://pubmed.ncbi.nlm.nih.gov/23339168/

カスタマーフィードバック

: Data from [Data independently produced by , , Liver Transpl, 2016, 22(12):1697-1709]

: Data from [Data independently produced by , , PLOS ONE, 2016, 11(3): e0152120.]

: Data from [Data independently produced by , , J Cell Biochem, 2018, 119(4):3440-3450]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

BRD7 Inhibited Immune Escape in Nasopharyngeal Carcinoma via Inhibiting PD-L1 Expression [ Int J Biol Sci, 2025, 21(5):1914-1931]	PubMed: 40083706
Role of IL-6/STAT3 Axis in Resistance to Cisplatin in Gastric Cancers [ Biomedicines, 2023, 11(3)694]	PubMed: 36979673
Curcumin Analog, HO-3867, Induces Both Apoptosis and Ferroptosis via Multiple Mechanisms in NSCLC Cells with Wild-Type p53 [ Evid Based Complement Alternat Med, 2023, 2023:8378581]	PubMed: 36814470
The regulation of T helper cell polarization by the diterpenoid fraction of Rhododendron molle based on the JAK/STAT signaling pathway [ Front Pharmacol, 2022, 13:1039441]	PubMed: 36386123
The regulation of T helper cell polarization by the diterpenoid fraction of Rhododendron molle based on the JAK/STAT signaling pathway [ Front Pharmacol, 2022, 13:1039441]	PubMed: 36386123
Establishment and characterization of immortalized sweat gland myoepithelial cells [ Sci Rep, 2022, 12(1):7]	PubMed: 34997030
Tumor Environment Promotes Lnc57Rik-Mediated Suppressive Function of Myeloid-Derived Suppressor Cells [ J Immunol, 2022, 209(7):1401-1413]	PubMed: 36038289
IL-27 signalling promotes adipocyte thermogenesis and energy expenditure [ Nature, 2021, 600(7888):314-318]	PubMed: 34819664
Gene deficiency or pharmacological inhibition of PDCD4-mediated FGR signaling protects against acute kidney injury [ Acta Pharm Sin B, 2021, 11(2):394-405]	PubMed: 33643819
Surfactant protein A modulates the activities of the JAK/STAT pathway in suppressing Th1 and Th17 polarization in murine OVA-induced allergic asthma [ Lab Invest, 2021, 10.1038/s41374-021-00618-1]	PubMed: 34108631

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。