Hoechst 33342

製品コードS0485 バッチS048501

化学情報

	Synonyms	Pibenzimol, bisBenzimide H 33342, HOE 33342, HO342	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₂₇H₂₈N₆O
	分子量	452.55	CAS No.	23491-52-3
Solubility (25°C)*	体外	Water	90 mg/mL (198.87 mM)
		DMSO	10 mg/mL (22.09 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Hoechst 33342 (Pibenzimol, bisBenzimide H 33342, HOE 33342, HO342) is a membrane-permeant fluorescent stain that can stain live cells. Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove and greatly increases the fluorescence. It allows easy visualization of the nucleus in interphase cells and chromosomes in mitotic cells.
in vitro	1.1 Preparation of the stock solution Dissolve 10 mg of Hoechst in 5 mL DMSO Note: It is recommended to store the stock solution at 4℃ or -20℃ away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of Hoechst working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain final concentration 10 μg/mL Hoechst working solution. Note: Please adjust the concentration of Hoechst working solution according to the actual situation. 1.Cell staining 2.1 Suspension cells（6-well plate） a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106/mL. b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes. c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant. d. Wash twice with PBS, 5 minutes each time. e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a. Culture adherent cells on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes. d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

製品説明

Hoechst 33342 (Pibenzimol, bisBenzimide H 33342, HOE 33342, HO342) is a membrane-permeant fluorescent stain that can stain live cells. Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove and greatly increases the fluorescence. It allows easy visualization of the nucleus in interphase cells and chromosomes in mitotic cells.

in vitro

1.1 Preparation of the stock solution
Dissolve 10 mg of Hoechst in 5 mL DMSO
Note: It is recommended to store the stock solution at 4℃ or -20℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Hoechst working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain final concentration 10 μg/mL Hoechst working solution.
Note: Please adjust the concentration of Hoechst working solution according to the actual situation.
1.Cell staining
2.1 Suspension cells（6-well plate）
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

プロトコル(参考用のみ)

参考

[1] Brad Chazotte. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.

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