Homoharringtonine (HHT)

製品コードS9015 バッチS901504

印刷

化学情報

Chemical Structure Synonyms CGX-635, Omacetaxine mepesuccinate, Myelostat, NSC 141633 Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C29H39NO9
分子量 545.62 CAS No. 26833-87-4
Solubility (25°C)* 体外 DMSO 100 mg/mL (183.27 mM)
Ethanol 100 mg/mL (183.27 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Homoharringtonine (HHT), a plant alkaloid with antitumor properties, inhibits protein translation by preventing the initial elongation step of protein synthesis via an interaction with the ribosomal A-site. Homoharringtonine reversiblely inhibits IL-6-induced STAT3 Tyrosine 705 phosphorylation and reduced anti-apoptotic proteins expression.
in vitro

Homoharringtonine (HHT), a plant alkaloid with antitumor properties, induces p62-mediated autophagy in imatinib-resistant CML K562G cells, thus promoting autophagic degradation of the BCR-ABL protein.[1]
in vivo

Homoharringtonine (HHT) in combination with ABT-199, significantly prolongs overall survival of primary diffuse large B-cell lymphoma (DLBCL) xenograft-bearing mice compared with single-agent approaches.[2]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 K562 cells, K562G cells
濃度 100, 50, 25, 12.5, 6.2, 3.1, 1.56, 0.78 and 0.39 ng/ml
反応時間 48 h
実験の流れ

K562, K562G, and siRNA-K562G cells are seeded in 384-well plates at a density of 8,000 cells/well, and then treated with 0.1% DMSO (vehicle control) or HHT at concentrations of 100, 50, 25, 12.5, 6.2, 3.1, 1.56, 0.78 and 0.39 ng/ml for 48 h. According to the manufacturer's instructions, cell proliferation is assayed using an MTS assay, and the absorbance is measured.
動物実験 動物モデル Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice of xenograft models of DLBCL
投薬量 50 µg/dose
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473] PubMed: 37833415
USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473] PubMed: 37833415
TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment [ iScience, 2023, 26(4):106543] PubMed: 37123244
Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency [ Autophagy, 2022, 1-19] PubMed: 35287545
The therapeutic effect of KSP inhibitors in preclinical models of cholangiocarcinoma [ Cell Death Dis, 2022, 13-9:799] PubMed: 36123339
A survey of optimal strategy for signature-based drug repositioning and an application to liver cancer [ Elife, 2022, 11e71880] PubMed: 35191375
SiCoDEA: A Simple, Fast and Complete App for Analyzing the Effect of Individual Drugs and Their Combinations [ Biomolecules, 2022, 12(7)904] PubMed: 35883460
Establishment and Characterization of NCC-PMP1-C1: A Novel Patient-Derived Cell Line of Metastatic Pseudomyxoma Peritonei [ J Pers Med, 2022, 12(2)258] PubMed: 35207746
Homoharringtonine Attenuates Dextran Sulfate Sodium-Induced Colitis by Inhibiting NF-κB Signaling [ Mediators Inflamm, 2022, 2022:3441357] PubMed: 36211988
Establishment and characterization of NCC-UPS4-C1: a novel cell line of undifferentiated pleomorphic sarcoma from a patient with Li-Fraumeni syndrome [ Hum Cell, 2022, 10.1007/s13577-022-00671-y] PubMed: 35118583

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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