Homoharringtonine (HHT)

製品コードS9015 バッチS901507

印刷

化学情報

Synonyms

CGX-635, Myelostat, NSC 141633

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₂₉H₃₉NO₉

分子量

545.62

CAS No.

26833-87-4

Solubility (25°C)*

体外

DMSO

100 mg/mL (183.27 mM)

Ethanol

25 mg/mL (45.81 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Homoharringtonine (HHT), a plant alkaloid with antitumor properties, inhibits protein translation by preventing the initial elongation step of protein synthesis via an interaction with the ribosomal A-site. Homoharringtonine reversiblely inhibits IL-6-induced STAT3 Tyrosine 705 phosphorylation and reduced anti-apoptotic proteins expression.
in vitro	Homoharringtonine (HHT), a plant alkaloid with antitumor properties, induces p62-mediated autophagy in resistant CML K562G cells, thus promoting autophagic degradation of the BCR-ABL protein.^[1]
in vivo	In combination with ABT-199, Homoharringtonine (HHT) significantly prolongs overall survival of primary diffuse large B-cell lymphoma (DLBCL) xenograft-bearing mice compared with single-agent approaches.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	K562 cells, K562G cells
	濃度	100, 50, 25, 12.5, 6.2, 3.1, 1.56, 0.78 and 0.39 ng/ml
	反応時間	48 h
	実験の流れ	Homoharringtonine (HHT) was used to treat K562, K562G, and siRNA-K562G cells seeded in 384-well plates at a density of 8,000 cells/well, at concentrations of 100, 50, 25, 12.5, 6.2, 3.1, 1.56, 0.78 and 0.39 ng/ml for 48 h, with 0.1% DMSO as a vehicle control. According to the manufacturer's instructions, cell proliferation is assayed using an MTS assay, and the absorbance is measured.
動物実験	動物モデル	Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ mice of xenograft models of DLBCL
	投薬量	50 µg/dose
	投与方法	i.p.

参考

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6908937/
https://pubmed.ncbi.nlm.nih.gov/26467384/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Oncogenic role of RARG rearrangements in acute myeloid leukemia resembling acute promyelocytic leukemia [ Nat Commun, 2025, 16(1):617]	PubMed: 39805831
HMGA2 and protein leucine methylation drive pancreatic cancer lineage plasticity [ Nat Commun, 2025, 16(1):4866]	PubMed: 40419509
Patient-derived rhabdomyosarcoma cells recapitulate the genetic and transcriptomic landscapes of primary tumors [ iScience, 2024, 27(10):110862]	PubMed: 39319271
Comprehensive Analysis of the Tumour Immune Microenvironment in Canine Urothelial Carcinoma Reveals Immunosuppressive Mechanisms Induced by the COX-Prostanoid Cascade [ Vet Comp Oncol, 2024, 10.1111/vco.12999]	PubMed: 39179510
USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473]	PubMed: 37833415
USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473]	PubMed: 37833415
TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment [ iScience, 2023, 26(4):106543]	PubMed: 37123244
Targeting HOXA in engineered iPSCs and pre-clinical leukaemia models [ ProQuest, 2023, 32089491]	PubMed: None
Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency [ Autophagy, 2022, 1-19]	PubMed: 35287545
The therapeutic effect of KSP inhibitors in preclinical models of cholangiocarcinoma [ Cell Death Dis, 2022, 13-9:799]	PubMed: 36123339

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。