HQNO

製品コードS8970 バッチS897001

化学情報

	Synonyms	2-heptyl-4-hydroxyquinoline-N-oxide	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₁₆H₂₁NO₂
	分子量	259.34	CAS No.	341-88-8
Solubility (25°C)*	体外	Ethanol	16 mg/mL (61.69 mM)
		DMSO	9 mg/mL (34.7 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	HQNO (2-heptyl-4-hydroxyquinoline-N-oxide) is a natural quinolone secreted by P. aeruginosa. HQNO is a potent inhibitor of the electron transport chain with Kd of 64 nM for the ubiquinol-ferricytochrome c oxidoreductase segment (Complex III) of the respiratory chain. HQNO is also an inhibitor of bacterial and mitochondrialType II NADH : quinone oxidoreductase (NDH-2) with IC50 of 7.3 μM in the presence of 50μM menadione.
in vitro	The aerobic respiratory chain-linked NADH oxidase system of B. cereus KCTC 3674 possesses an HQNO-sensitive NADH:quinone oxidoreductase that lacks an energy coupling site containing FAD as a cofactor (designated NDH-2). The respiratory inhibitor HQNO inhibits the activity of NADH:ubiquinone-1 oxidoreductase and NADH:menadione oxidoreductase by about 40% and 45%, respectively, at a concentration of 20 μM.^[3]

製品説明

HQNO (2-heptyl-4-hydroxyquinoline-N-oxide) is a natural quinolone secreted by P. aeruginosa. HQNO is a potent inhibitor of the electron transport chain with Kd of 64 nM for the ubiquinol-ferricytochrome c oxidoreductase segment (Complex III) of the respiratory chain. HQNO is also an inhibitor of bacterial and mitochondrialType II NADH : quinone oxidoreductase (NDH-2) with IC50 of 7.3 μM in the presence of 50μM menadione.

in vitro

The aerobic respiratory chain-linked NADH oxidase system of B. cereus KCTC 3674 possesses an HQNO-sensitive NADH:quinone oxidoreductase that lacks an energy coupling site containing FAD as a cofactor (designated NDH-2). The respiratory inhibitor HQNO inhibits the activity of NADH:ubiquinone-1 oxidoreductase and NADH:menadione oxidoreductase by about 40% and 45%, respectively, at a concentration of 20 μM.^[3]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Bacillus cereus KCTC 3674
	濃度	10 μM, 20 μM, 30 μM, 40 μM, 50 μM
	反応時間	--
	実験の流れ	The assay mixture of NADH:ubiquinone-1 oxidoreductase containes a Triton X-100 extract of B. cereus membranes (10 μg protein), 30 mM KCN, and 50 μM Q-1 in 2 ml of 50 mM MES-KOH (pH 6.0) containing 0.1M NaCl. The activity of NADH: ubiquinone-1 oxidoreductase is measured with various concentrations of HQNO.

参考

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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