Hydroxytyrosol

製品コードS3826 バッチS382602

印刷

化学情報

Chemical Structure Synonyms 3-Hydroxytyrosol; 3,4-dihydroxyphenylethanol; Dihydroxyphenylethanol; 2-(3,4-Di-hydroxyphenyl)-ethanol Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C8H10O3
分子量 154.16 CAS No. 10597-60-1
Solubility (25°C)* 体外 DMSO 31 mg/mL (201.08 mM)
Water 31 mg/mL (201.08 mM)
Ethanol 31 mg/mL (201.08 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Hydroxytyrosol (3,4-dihydroxyphenylethanol) is one of the main phenolic components of olive oil with excellent antioxidant, antimicrobial and anticarcinogenic activities.
in vitro Hydroxytyrosol decreases the intracellular reactive oxygen species (ROS) level in MCF10A cells but not in MCF7 or MDA-MB-231 cells. Hydroxytyrosol prevents oxidative DNA damage in the three breast cell lines: MCF10A, MDA-MB-231 and MCF7 cells. It is a potent antioxidant because of its marked antioxidant activity, its ability to scavenge oxygen and nitrogen free radicals, to inhibit Low Density Lipoprotein (LDL) oxidation, platelet aggregation and endothelial cell activation and its protection against DNA damage. Hydroxytyrosol is able to reduce the synthesis of prostaglandin E2 blocking the transcription of COX-2 and 5-lipooxygenase, thereby reducing the chronic influence associated with diseases such as cancer[1].
in vivo HT administration protects against hepatic I/R injury, as indicated by the decreased levels of serum aminotransferase and less parenchymal necrosis and apoptosis. Serum levels of tumor necrosis factor-α, interleukin-6, macrophage inflammatory protein 2, as well as reactive oxygen species (ROS) content in liver tissues, are all decreased by HT[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 MDA-MB-231, MCF10A and MCF7 cells
濃度 1 to 100 μM
反応時間 24 hours
実験の流れ Cells are seeded at 2 × 103 cells/well (MCF7) or 1 × 103 cells/well (MDA-MB-231 and MCF10A) into 96-well culture plates (flat bottom) (100 μL of cell suspension/well). At 24 h after plating, 100 μL of fresh culture medium, with different concentrations of HT is added in triplicate to the wells. Plates are incubated for 24 h or 24 h followed by a 48 h proliferation period with fresh medium at 37 °C and 5% CO2. At these time points, medium is removed and 200 μL of fresh RPMI medium without phenol red that contained XTT (200 μg/mL) and PMS (20 μg/mL) is added. Plates are incubated for 3 h at 37 °C in 5% CO2 and absorbance is measured at 450 nm wavelength (620 nm as reference) in a plate reader.
動物実験 動物モデル A mouse model of partial hepatic I/R injury (Male BALB/c mice)
投薬量 1 or 10 mg/kg
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Hydroxytyrosol Recovers SARS-CoV-2-PLpro-Dependent Impairment of Interferon Related Genes in Polarized Human Airway, Intestinal and Liver Epithelial Cells [ Antioxidants (Basel), 2022, 11(8)1466] PubMed: 36009185
Combination Treatment with Hydroxytyrosol and Vitamin E Improves NAFLD-Related Fibrosis [ Nutrients, 2022, 14(18)3791] PubMed: 36145170

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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