I3MT-3

製品コードS3541 バッチS354101

印刷

化学情報

Synonyms

HMPSNE

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₁₇H₁₄N₂O₂S

分子量

310.37

CAS No.

459420-09-8

Solubility (25°C)*

体外

DMSO

62 mg/mL (199.76 mM)

Ethanol

2 mg/mL (6.44 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	2.800mg/ml (9.02mM)	Taking the 1 mL working solution as an example, add 50 μL of 56 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	I3MT-3 (HMPSNE, compound 3) is a potent, selective, and cell-membrane permeable inhibitor of 3-mercaptopyruvate sulfurtransferase (3MST) with IC50 of 2.7 μM. I3MT-3 targets a persulfurated cysteine residue located in the active site of 3MST.
in vitro	I3MT-3 (HMPSNE, compound 3), a pharmacological 3-MST inhibitor, exerts a bell-shaped effect on several cellular bioenergetic parameters related to oxidative phosphorylation in CT26 cells, suggesting that the 3-MST/H2S system significantly contributes to the maintenance of cell proliferation, migration and cellular bioenergetics.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	CT26 mouse colon carcinoma cell line
	濃度	1-300 μM
	反応時間	24 h
	実験の流れ	The activity of 3-MST in presence of various concentrations of HMPSNE was also tested in CT26 cells homogenates. CT26 cells were centrifuged 5 min at 400× g in order to resuspend the pellet in lysis buffer 150 mM NaCl and 50 mM Tris-HCl, pH 8 containing 1% NP40 and Protease and Phosphatase Inhibitor Cocktail added just before use. Samples were sonicated with ultrasound probe three times a cycle of 5 s on and 5 s off at 70% amplitude and kept on ice for 30 min. The protein concentrations were measured as described above. This compound dilutions were prepared as above and incubated 24 h at 37 °C with 100 µg/well of proteins from CT26 homogenates in black 96-well plate. After treating the homogenate with this chemical, 3-mercaptopyruvate was added for a final concentration of 500 µM and AzMC for a final concentration of 10 µM. Fluorescence measurement and analysis were performed.

参考

https://pubmed.ncbi.nlm.nih.gov/28079151/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175125/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Ergothioneine boosts mitochondrial respiration and exercise performance via direct activation of MPST [ bioRxiv, 2024, 2024.04.10.588849]	PubMed: 38645260

Ergothioneine boosts mitochondrial respiration and exercise performance via direct activation of MPST [ bioRxiv, 2024, 2024.04.10.588849]

PubMed: 38645260

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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