IDF-11774

製品コードS8771 バッチS877101

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₂₃H₃₂N₂O₂

分子量

368.51

CAS No.

1429054-28-3

Solubility (25°C)*

体外

DMSO

15 mg/mL (40.7 mM)

Ethanol

5 mg/mL (13.56 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

10%DMSO 1 40%PEG300 2 5%Tween80 3 45%ddH2O

3.0mg/ml

Taking the 1 mL working solution as an example, add 100 μL of 30 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 450 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	IDF-11774 is a hypoxia-inducible factor-1 (HIF-1) inhibitor. It reduces the HRE-luciferase activity of HIF-1α (IC50 = 3.65 μM) and blocks HIF-1α accumulation under hypoxia in HCT116 human colon cancer cells.
in vitro	IDF-11774 inhibits the accumulation of HIF-1α in vitro and in vivo in colorectal carcinoma HCT116 cells under hypoxic conditions. The treatment of IDF-11774 suppresses angiogenesis of cancer cells by reducing the expression of HIF-1 target genes, reduces glucose uptake, thereby sensitizing cells to growth under low glucose conditions, and decreases the extracellular acidification rate (ECAR) and oxygen consumption rate of cancer cells. Therefore, IDF-11774 reduces cancer cell growth through the regulation of cancer glycolytic metabolism and energy production^[1].
in vivo	IDF-11774 exhibited substantial anticancer efficacy in mouse models containing KRAS, PTEN, or VHL mutations, which often occur in malignant cancers^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HCT116 cells
	濃度	5, 10, 20 μM
	反応時間	18 h
	実験の流れ	--
動物実験	動物モデル	Balb/c nude mice bearing tumors (100 mm³) formed from HCT116 cells (1 × 10⁷) expressing HRE-luciferase by subcutaneous injection
	投薬量	50 mg/kg
	投与方法	p.o.

参考

[1] Ban HS, et al. Cell Death Dis. 2017, 8(6):e2843.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Targeting APLN/APJ restores blood-testis barrier and improves spermatogenesis in murine and human diabetic models [ Nat Commun, 2022, 13(1):7335]	PubMed: 36443325
Staphylococcus aureus Infection Initiates Hypoxia-Mediated Transforming Growth Factor-β1 Upregulation to Trigger Osteomyelitis [ mSystems, 2022, 7(4):e0038022]	PubMed: 35852344
Trabectedin induces ferroptosis via regulation of HIF-1α/IRP1/TFR1 and Keap1/Nrf2/GPX4 axis in non-small cell lung cancer cells [ Chem Biol Interact, 2022, 369:110262]	PubMed: 36396105
Inhibition of hypoxia-inducible factor-1α alleviates acinar cell necrosis in a mouse model of acute pancreatitis [ Biochem Biophys Res Commun, 2021, 572:72-79]	PubMed: 34358966
Secreted frizzled-related protein 2 inhibits the proliferation and metastasis of human colorectal cancer cell line SW480 [ Basic & Clinical Medicine, 2020, 40(9): 1212-1217]	PubMed: None

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。