IKE(Imidazole ketone erastin)

製品コードS8877 バッチS887703

印刷

化学情報

Chemical Structure Synonyms PUN30119 Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C35H35ClN6O5
分子量 655.14 CAS No. 1801530-11-9
Solubility (25°C)* 体外 DMSO 100 mg/mL (152.63 mM)
Ethanol 100 mg/mL (152.63 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO Corn oil
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 IKE(Imidazole ketone erastin) is a potent, selective, and metabolically stable system xc– inhibitor and inducer of ferroptosis.
in vitro

Imidazole ketone erastin (IKE) is an erastin analog with nanomolar potency, high metabolic stability, and intermediate water solubility. Its treatment potently reduces DLBCL cell number[1].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 DLBCL cell line
濃度 0-100 μM
反応時間 24 h
実験の流れ

DLBCL cells are plated at 10,000 cells per well in white 384-well plates (32 μL per well) in technical duplicates and incubated overnight. The cells are then treated with 8 μL medium containing a two-fold dilution series of vehicle (DMSO), IKE (starting from 100 μM) with or without Fer-1 (starting from 200 μM). After 24 h incubation, 40 μL of 50% CellTiter-Glo / 50% cell culture medium is added to each well and incubated at room temperature with shaking for 15 min. Luminescence is measured. All cell viability data are normalized to the DMSO vehicle condition. Experiments are performed three independent times with different passages for each cell line. From these data, dose-response curves and IC50 values are computed.
動物実験 動物モデル Non-obese diabetic/ severe combined immunodeficiency (NOD/SCID) mice
投薬量 50 mg/kg
投与方法 IP, IV and oral administration

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

CYLD regulates cell ferroptosis through Hippo/YAP signaling in prostate cancer progression [ Cell Death Dis, 2024, 15(1):79] PubMed: 38246916
Intermittent dietary methionine deprivation facilitates tumoral ferroptosis and synergizes with checkpoint blockade [ Nat Commun, 2023, 14(1):4758] PubMed: 37553341
Intermittent dietary methionine deprivation facilitates tumoral ferroptosis and synergizes with checkpoint blockade [ Nat Commun, 2023, 14(1):4758] PubMed: 37553341
RB1-deficient prostate tumor growth and metastasis are vulnerable to ferroptosis induction via the E2F/ACSL4 axis [ J Clin Invest, 2023, 133(10)e166647] PubMed: 36928314
RB1-deficient prostate tumor growth and metastasis are vulnerable to ferroptosis induction via the E2F/ACSL4 axis [ J Clin Invest, 2023, 133(10)e166647] PubMed: 36928314
Targeting N-glycosylation of 4F2hc mediated by glycosyltransferase B3GNT3 sensitizes ferroptosis of pancreatic ductal adenocarcinoma [ Cell Death Differ, 2023, 30(8):1988-2004] PubMed: 37479744
AMER1 deficiency promotes the distant metastasis of colorectal cancer by inhibiting SLC7A11- and FTL-mediated ferroptosis [ Cell Rep, 2023, 42(9):113110] PubMed: 37682704
Histone acetyltransferase P300 deficiency promotes ferroptosis of vascular smooth muscle cells by activating the HIF-1α/HMOX1 axis [ Mol Med, 2023, 29(1):91] PubMed: 37415103
ZNF498 promotes hepatocellular carcinogenesis by suppressing p53-mediated apoptosis and ferroptosis via the attenuation of p53 Ser46 phosphorylation [ J Exp Clin Cancer Res, 2022, 41(1):79] PubMed: 35227287
BRD4770 functions as a novel ferroptosis inhibitor to protect against aortic dissection [ Pharmacol Res, 2022, 177:106122] PubMed: 35149187

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。