IKE (Imidazole Ketone Erastin)

製品コードS8877 バッチS887702

印刷

化学情報

Synonyms

PUN30119

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₃₅H₃₅ClN₆O₅

分子量

655.14

CAS No.

1801530-11-9

Solubility (25°C)*

体外

DMSO

100 mg/mL (152.63 mM)

Ethanol

100 mg/mL (152.63 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	IKE(Imidazole ketone erastin) is a potent, selective, and metabolically stable system xc– inhibitor and inducer of ferroptosis.
in vitro	Imidazole ketone erastin (IKE) is an erastin analog with nanomolar potency, high metabolic stability, and intermediate water solubility. Its treatment potently reduces DLBCL cell number^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	DLBCL cell line
	濃度	0-100 μM
	反応時間	24 h
	実験の流れ	DLBCL cells are plated at 10,000 cells per well in white 384-well plates (32 μL per well) in technical duplicates and incubated overnight. The cells are then treated with 8 μL medium containing a two-fold dilution series of vehicle (DMSO), IKE (starting from 100 μM) with or without Fer-1 (starting from 200 μM). After 24 h incubation, 40 μL of 50% CellTiter-Glo / 50% cell culture medium is added to each well and incubated at room temperature with shaking for 15 min. Luminescence is measured. All cell viability data are normalized to the DMSO vehicle condition. Experiments are performed three independent times with different passages for each cell line. From these data, dose-response curves and IC50 values are computed.
動物実験	動物モデル	Non-obese diabetic/ severe combined immunodeficiency (NOD/SCID) mice
	投薬量	50 mg/kg
	投与方法	IP, IV and oral administration

参考

https://pubmed.ncbi.nlm.nih.gov/30799221/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

SLC7A11 is an unconventional H+ transporter in lysosomes [ Cell, 2025, S0092-8674(25)00406-4]	PubMed: 40280132
Endoplasmic reticulum-mitochondria contacts are prime hotspots of phospholipid peroxidation driving ferroptosis [ Nat Cell Biol, 2025, 27(6):902-917]	PubMed: 40514428
BDH2-driven lysosome-to-mitochondria iron transfer shapes ferroptosis vulnerability of the melanoma cell states [ Nat Metab, 2025, 7(9):1851-1870]	PubMed: 40957995
Targeting pancreatic cancer glutamine dependency confers vulnerability to GPX4-dependent ferroptosis [ Cell Rep Med, 2025, 6(2):101928]	PubMed: 39879992
Vorapaxar enhanced mitochondria-associated ferroptosis primes cancer immunotherapy via targeting FOXO1/HMOX1 axis [ Cell Rep Med, 2025, 6(10):102371]	PubMed: 41005298
Neutralization of acyl coenzyme A binding protein for the experimental prevention and treatment of hepatocellular carcinoma [ Cell Rep Med, 2025, 6(7):102232]	PubMed: 40628264
SLC25A1 and ACLY maintain cytosolic acetyl-CoA and regulate ferroptosis susceptibility via FSP1 acetylation [ EMBO J, 2025, 10.1038/s44318-025-00369-5]	PubMed: 39881208
Periplocin potentiates ferroptotic cell death in non-small cell lung cancer by inducing the degradation of Nrf2 [ Cancer Cell Int, 2025, 25(1):294]	PubMed: 40760694
Mitochondrial phosphoenolpyruvate carboxykinase 2 counteracts ferroptosis via catalytic activity independent of mitochondrial stress [ Biochem Biophys Res Commun, 2025, 778:152383]	PubMed: 40712389
Targeting lipid scrambling potentiates ferroptosis and triggers tumor immune rejection [ Sci Adv, 2025, 11(33):eadx6587]	PubMed: 40815641

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。