Imperatorin

製品コードS3809 バッチS380901

印刷

化学情報

Synonyms

Ammidin, Marmelosin, Pentosalen, 8-Isoamylenoxypsoralen, 8-Isopentenyloxypsoralene

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₆H₁₄O₄

分子量

270.28

CAS No.

482-44-0

Solubility (25°C)*

体外

DMSO

54 mg/mL (199.79 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Imperatorin (Ammidin, Marmelosin, Pentosalen, 8-Isoamylenoxypsoralen), a naturally occurring psoralen-type furanocoumarin, is a potent myorelaxant agent and acts as a calcium antagonist on vascular smooth muscle.
in vitro	Imperatorin has estrogen-like activities in MCF-7 cells and stimulatory effects on ALP activity of Saos-2 osteoblast cells^[1].
in vivo	Imperatorin acutely administered at the doses of 10 and 20 mg/kg impairs anxiogenic effect of nicotine in the elevated plus maze (EPM) test. This compound acts as irreversible inactivator of GABA-transaminase and increases the GABA content in the brain. Co-administration of subthreshold doses of this chemical and nicotine produces an improvement inmemory processes in animals^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	MCF-7 cells
	濃度	0.1 μmol/L
	反応時間	24 h
	実験の流れ	The MCF-7 cells are seeded into six-well plates in standard growth medium at a density of 100 000 cells per well and allowed to attach overnight (24 h). The growth medium is replaced with phenol red free DMEM containing 10% charcoal-dextran stripped human serum (hormone-free) for 72 h, after which the medium is refreshed and this chemical is added. After a further 24-h exposure, the cells are harvested and washed with PBS. Cell pellets, which are obtained by centrifugation at 1000 rpm for 15 minutes, are stained with 1 mL of freshly propidium iodide (PI) staining solution (0.1% of Triton X-100, 0.1% of sodium citrate, 50 μg/mL of PI and 10 μg/mL of RNase A in PBS). Flow cytometric analysis of cell cycle distribution is performed.
動物実験	動物モデル	male Swiss mice
	投薬量	1, 10 and 20 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/27473958/
https://pubmed.ncbi.nlm.nih.gov/23999469/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Imperatorin Restores Chemosensitivity of Multidrug-Resistant Cancer Cells by Antagonizing ABCG2-Mediated Drug Transport [ Pharmaceuticals (Basel), 2023, 10.3390/ph16111595]	PubMed: 38004460
Coumarins of Lovage Roots (Levisticum officinale W.D.J.Koch): LC-MS Profile, Quantification, and Stability during Postharvest Storage [ Metabolites, 2022, 13(1)3]	PubMed: 36676932
Direct Targeting of CREB1 with Imperatorin Inhibits TGF β 2-ERK Signaling to Suppress Esophageal Cancer Metastasis [ Adv Sci (Weinh), 2020, 7(16):2000925]	PubMed: 32832354
Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function [ Front Cell Dev Biol, 2020, 8:592433]	PubMed: 33409275
Imperatorin improves in vitro porcine embryo development by reducing oxidative stress and autophagy. [ Theriogenology, 2019, 10.1016/j.theriogenology]	PubMed: 31831188

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。