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Synonyms | IA, IAM, 2-Iodoacetamide | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C2H4INO |
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分子量 | 184.96 | CAS No. | 144-48-9 | |
Solubility (25°C)* | 体外 | DMSO | 40 mg/mL (216.26 mM) | |
Water | 40 mg/mL (216.26 mM) | |||
Ethanol | 40 mg/mL (216.26 mM) | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Iodoacetamide (IA, IAM, 2-Iodoacetamide) is an alkylating agent that is commonly used for alkylation of cysteine during sample preparation for proteomics. |
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in vitro | A multiple cleavage cascade mechanism is partially utilized to produce virus structural proteins. In infected cells, the largest viral induced protein obtained has a molecular weight of 144,000. Pretreatment of the infected cells with iodoacetamide before pulsing with 35S-methionine results in the appearance of two polypeptides of molecular weights 205,000 and 190,000. Other changes occur in viral protein precursors in the presence of iodoacetamide.[2] |
in vivo | Iodoacetamide induces a concentration-dependent weight loss and reduces water intake in both species (Wistar rats and CD1, C57Bl/6J mice). Iodoacetamide induces gastritis in rats.[3] |
細胞アッセイ | 細胞株 | Drosophila melanogaster cells |
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濃度 | 2 mM | |
反応時間 | 15 mins | |
実験の流れ | Infected cells pretreated with 2 mM iodoaeetamide for 15 minutes before pulsing with 500 μCi of 35S-methionine for 30 minutes. The molecular weights are calculated by direct comparison of the stained dried slab gel and the corresponding autoradiogram. |
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動物実験 | 動物モデル | Wistar rats, 8-week-old male CD1 and C57Bl/6J mice |
投薬量 | 0.05%, 0.1%, 0.2%, 0.3% and 0.5% concentration | |
投与方法 | Oral gavage |
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The compound millepachine and its derivatives inhibit tubulin polymerization by irreversibly binding to the colchicine-binding site in β-tubulin [ J Biol Chem, 2018, 293(24):9461-9472] | PubMed: 29691282 |
Global MS-Based Proteomics Drug Profiling [ Methods Mol Biol, 2016, 1449:469-79] | PubMed: 27613057 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。