Iodoacetamide

製品コードS9827 バッチS982701

印刷

化学情報

Synonyms

IA, IAM, 2-Iodoacetamide

Storage
(From the date of receipt)

3 years -20°C(in the dark) powder
1 year -80°C(in the dark) in solvent

化学式

C₂H₄INO

分子量

184.96

CAS No.

144-48-9

Solubility (25°C)*

体外

DMSO

40 mg/mL (216.26 mM)

Water

40 mg/mL (216.26 mM)

Ethanol

40 mg/mL (216.26 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	2mg/ml (10.81mM)	Taking the 1 mL working solution as an example, add 50 μL of 40 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.29mg/ml (1.57mM)	Taking the 1 mL working solution as an example, add 50 μL of 5.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Iodoacetamide (IA, IAM, 2-Iodoacetamide) is an alkylating agent that is commonly used for alkylation of cysteine during sample preparation for proteomics.
in vitro	A multiple cleavage cascade mechanism is partially utilized to produce virus structural proteins. In infected cells, the largest viral induced protein obtained has a molecular weight of 144,000. Pretreatment of the infected cells with iodoacetamide before pulsing with 35S-methionine results in the appearance of two polypeptides of molecular weights 205,000 and 190,000. Other changes occur in viral protein precursors in the presence of this compound.^[2]
in vivo	Iodoacetamide induces a concentration-dependent weight loss and reduces water intake in both species (Wistar rats and CD1, C57Bl/6J mice). This compound induces gastritis in rats.^[3]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Drosophila melanogaster cells
	濃度	2 mM
	反応時間	15 mins
	実験の流れ	Infected cells pretreated with 2 mM iodoaeetamide for 15 minutes before pulsing with 500 μCi of 35S-methionine for 30 minutes. The molecular weights are calculated by direct comparison of the stained dried slab gel and the corresponding autoradiogram.
動物実験	動物モデル	Wistar rats, 8-week-old male CD1 and C57Bl/6J mice
	投薬量	0.05%, 0.1%, 0.2%, 0.3% and 0.5% concentration
	投与方法	Oral gavage

参考

https://pubmed.ncbi.nlm.nih.gov/28799334/
https://pubmed.ncbi.nlm.nih.gov/6788024/
https://pubmed.ncbi.nlm.nih.gov/32764237/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

The compound millepachine and its derivatives inhibit tubulin polymerization by irreversibly binding to the colchicine-binding site in β-tubulin [ J Biol Chem, 2018, 293(24):9461-9472]	PubMed: 29691282
Global MS-Based Proteomics Drug Profiling [ Methods Mol Biol, 2016, 1449:469-79]	PubMed: 27613057

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。