Irinotecan hydrochloride

製品コードS5026 バッチS502604

印刷

化学情報

Chemical Structure Synonyms CPT-11 hydrochloride, (+)-Irinotecan hydrochloride Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C33H39ClN4O6
分子量 623.14 CAS No. 100286-90-6
Solubility (25°C)* 体外 DMSO 100 mg/mL (160.47 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
2% DMSO 40% PEG 300 5%Tween80 53%ddH2O
2.0mg/ml Taking the 1 mL working solution as an example, add 20 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 530 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
2%DMSO 98%Corn oil
0.24mg/ml Taking the 1 mL working solution as an example, add 20 μL of 12 mg/ml clear DMSO stock solution to 980 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Irinotecan (CPT-11, (+)-Irinotecan) hydrochloride is an inhibitor of Topoisomerase I (Topo I) that exhibits cytotoxicity in LoVo cells and HT-29 cells with IC50 of 15.8 μM and 5.17 μM, respectively.
in vitro

The combination of gefitinib and irinotecan has a significant synergistic effect on the proliferation and migration of MDA-MB-231 cells.[2]
in vivo

The combination of gefitinib and irinotecan has a significant synergistic effect in the treatment of TNBC subtype cells in xenograft model.[2]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Human BC cell lines
濃度 32 μM
反応時間 24 h, 72 h
実験の流れ

Cells are seeded in 96-well plates at 6 × 103 cells/well for overnight incubation. The cells are exposed to irinotecan alone or combinations. Seventy-two hours after drug treatment, cell viability is tested with the MTT assay. For wound fealing assay, cells are seeded in 6-well culture plates at a density of 3 × 105 cells/mL. After growing to confluence, cells are treated with irinotecan for 48 h in DMEM.
動物実験 動物モデル Female BALB/c nude mice bearing MDA-MB-231 cells
投薬量 8 mg/kg
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

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Integrated genome and tissue engineering enables screening of cancer vulnerabilities in physiologically relevant perfusable ex vivo cultures [ Biomaterials, 2021, 280:121276] PubMed: 34890975

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。