ISO-1

製品コードS7732 バッチS773206

化学情報

	Synonyms	MIF Antagonist	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₁₂H₁₃NO₄
	分子量	235.24	CAS No.	478336-92-4
Solubility (25°C)*	体外	DMSO	47 mg/mL (199.79 mM)
		Ethanol	47 mg/mL (199.79 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	ISO-1（MIFアンタゴニスト）は、IC50が約7 μMのMIF（マクロファージ遊走阻止因子）D-ドーパクロム異性化酵素活性の阻害剤です。
in vitro	ISO-1 significantly inhibits the cytokine activity in vitro. It inhibits tumor necrosis factor release from macrophages isolated from LPStreated wild type mice but has no effect on cytokine release from MIF-deficient macrophages.
in vivo	ISO-1 significantly increases the survival rate in severe sepsis induced by endotoxin or cecal ligation and puncture (CLP). It could prevent airway hyperresponsiveness (AHR) in mouse ovalbumin (OVA)-challenge models. This compound attenuated ozone-induced cell recruitment, cytokine release and AHR in the mouse but did not affect measures of emphysema. It protects photoreceptors and reduces gliosis in experimental retinal detachment.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	RAW 267.4 macrophages
	濃度	1-100 μM
	反応時間	30 min
	実験の流れ	RAW 267.4 macrophages were treated with various concentrations of ISO-1 (1-100 μM) 30 min prior to LPS addition. Macrophages were collected 2 h after activation with LPS and washed one time in PBS (pH 7.4). Nuclear extract was isolated using NE-PER Nuclear and Cytoplasmic Extraction Reagents.
動物実験	動物モデル	Male Balb/C mice, ∼8 weeks old, subjectd to endotoxemia or cecal ligation and puncture (CLP)
	投薬量	3.5-35 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/19090668/
https://pubmed.ncbi.nlm.nih.gov/16115897/
https://pubmed.ncbi.nlm.nih.gov/26752192/

https://www.nature.com/articles/s41598-017-14298-9

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Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Pericyte signaling via soluble guanylate cyclase shapes the vascular niche and microenvironment of tumors [ EMBO J, 2024, 43(8):1519-1544]	PubMed: 38528180
MicroRNA-451 Regulates Angiogenesis in Intracerebral Hemorrhage by Targeting Macrophage Migration Inhibitory Factor [ Mol Neurobiol, 2024, 61(12):10481-10499]	PubMed: 38743209
MicroRNA-451 Regulates Angiogenesis in Intracerebral Hemorrhage by Targeting Macrophage Migration Inhibitory Factor [ Mol Neurobiol, 2024, 10.1007/s12035-024-04207-3]	PubMed: 38743209
Moxibustion of Zusanli (ST36) and Shenshu (BL23) alleviates the inflammation of rheumatoid arthritis in rats through regulating macrophage migration inhibitory factor/glucocorticoids signaling [ J Tradit Chin Med, 2024, 44(2):353-361]	PubMed: 38504541
Microphysiological head and neck cancer model identifies novel role of lymphatically secreted monocyte migration inhibitory factor in cancer cell migration and metabolism [ Biomaterials, 2023, 298:122136]	PubMed: 37178589
MIF is a critical regulator of mononuclear phagocytic infiltration in hepatocellular carcinoma [ iScience, 2023, 26(8):107273]	PubMed: 37520719
MIF Increases sFLT1 Expression in Early Uncomplicated Pregnancy and Preeclampsia [ Int J Mol Sci, 2023, 24(12)10050]	PubMed: 37373198
Therapeutic targeting of tumor spheroids in a 3D microphysiological renal cell carcinoma-on-a-chip system [ Neoplasia, 2023, 10.1016/j.neo.2023.100948]	PubMed: 37944353
Ischemic stroke protected by ISO-1 inhibition of apoptosis via mitochondrial pathway [ Sci Rep, 2023, 13(1):2788]	PubMed: 36797398
On the origin of metastases: Induction of pro-metastatic states after impending cell death via ER stress, reprogramming, and a cytokine storm [ Cell Rep, 2022, 38(10):110490]	PubMed: 35263600

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。