Isopsoralen

製品コードS3892 バッチS389202

印刷

化学情報

Synonyms

angelicin

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₁H₆O₃

分子量

186.16

CAS No.

523-50-2

Solubility (25°C)*

体外

DMSO

9 mg/mL (48.34 mM)

Ethanol

5 mg/mL (26.85 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Isopsoralen (Angelicin), also known as angelicin, is a constituent of roots and leaves of angelica with anti-inflammatory activity and regulates LPS-induced inflammation via inhibiting MAPK/NF-κB pathways. It also shows antiviral activity against gammaherpesviruses.
in vitro	Isopsoralen treatment stimulates the accumulation of cartilage nodules in a dose-dependent manner. This compound enhances the expressions of chondrogenic marker genes such as collagen II, collagen X, OCN, Smad4 and Sox9 in a time-dependent manner. Furthermore, it induces the activation of extracellular signal-regulated kinase (ERK) and p38 MAP kinase, but not that of c-jun N-terminal kinase (JNK). It significantly enhances the protein expression of BMP-2 in a time-dependent manner and mediates a chondromodulating effect by BMP-2 or MAPK signaling pathways^[2].
in vivo	Isopsoralen has significant osteoprotective effect in female and male mice with sex hormone deprivation. After administration of this compound for 8 weeks, Bone strength increases and trabecular bone microstructure improves^[1]. After intravenous administration to wistar rats, the elimination half-lives of this chemical is 5.35 h. The area under the curves of the tissues for it decrease in the following order: kidney > lung > liver > heart > spleen > brain. After oral administration to Wistar rats, the elimination half-lives of this compound is 5.56 h, and its bioavailability is 70.35%^[3].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	ATDC5 cells
	濃度	0-1 μM
	反応時間	24 h
	実験の流れ	ATDC5 cells are seeded in 96-well plate at a density of 5×10³ cells/well and incubated overnight in media supplemented with 10% FBS followed by treatment with various concentrations of isopsoralen. After 24h of incubation, cells are washed with phosphate-buffered saline (PBS) and then treated with media containing 100 μg/ml MTT for 2 h at 37℃. The cells are then washed with PBS, and dissolved in 200 μl of DMSO. The resulting solubilized purple formazan is quantified by measuring the absorbance at a wavelength of 540 nm using a spectrophotometer.
動物実験	動物モデル	ICR mice
	投薬量	10 mg/kg and 20 mg/kg
	投与方法	intragastrically

参考

https://www.hindawi.com/journals/bmri/2016/6869452/
https://pubmed.ncbi.nlm.nih.gov/24130927/
https://pubmed.ncbi.nlm.nih.gov/20195822/

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。