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Synonyms | Hirsutrin, 3-Glucosylquercetin, Quercetin 3-o-glucopyranoside | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C21H20O12 |
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分子量 | 464.38 | CAS No. | 482-35-9 | |
Solubility (25°C)* | 体外 | DMSO | 93 mg/mL (200.26 mM) | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Isoquercitrin (Hirsutrin, 3-Glucosylquercetin, Quercetin 3-o-glucopyranoside), a flavonoid compound with anticancer activity isolated from Bidens bipinnata L, is an inhibitor of Wnt/β-catenin that acts downstream of the β-catenin nuclear translocation. |
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in vitro | Isoquercitrin exerts inhibitory effect on Wnt/β-catenin signaling and no toxic effects on colon cancer cell lines. It supresses the proliferation of colon cancer cells but does not affect non-tumoral colon cells. Isoquercitrin inhibits cell migration in colon cancer cells, but has also no apparent effect on the non-tumor colon cells. Isoquercitrin inhibits the signaling downstream of β-catenin nuclear translocation as isoquercitrin reverses the effect of β-catenin S33A, which cannot be phosphorylated and avoids degradation[1]. |
in vivo | Isoquercitrin treatment significantly affects Xenopus embryo development, most likely by disturbing axial patterning. Isoquercitrin affects canonical Wnt signaling downstream of β-Catenin nucleus translocation[1]. In animals receiving isoquercitrin, eosinophil counts are lower in the bronchoalveolar lavage fluid (BALF), blood and lung pa-renchyma. Neutrophil counts in blood and IL-5 levels in lung homogenate are lower in isoquercitrin-treated mice. No alterations in mononuclear cell numbers are observed. Isoquercitrin also demonstrates anti-asthmatic activity by inhibiting of carbachol- and leukotriene D4-induced contraction in guinea pig airways[2]. |
細胞アッセイ | 細胞株 | HCT-116 cells |
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濃度 | 75 and 150 μM | |
反応時間 | 24 h | |
実験の流れ | HCT-116 cells are treated with flavonoid isoquercitrin for 24 h at concentrations of 75 and 150 μM. DMSO (used as a vehicle to solubilize the flavonoids) is added to control cultures at 1% v/v. To stimulate the Wnt pathway, cells are treated with Wnt3a-conditioned medium (CM) for 6 h at 25% of conditioned medium. Cells are co-treated with Wnt3a-CM and isoquercitrin for 6 h at 75 or 150 μM. | |
動物実験 | 動物モデル | a murine model of asthma (Female BALB/c mice) |
投薬量 | 15 mg/kg | |
投与方法 | by oral gavage |
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Single-cell transcriptomics reveal extracellular vesicles secretion with a cardiomyocyte proteostasis signature during pathological remodeling [ Commun Biol, 2023, 6(1):79] | PubMed: 36681760 |
Identification of the main flavonoids of Abelmoschus manihot (L.) medik and their metabolites in the treatment of diabetic nephropathy [ Front Pharmacol, 2023, 14:1290868] | PubMed: 38313075 |
Periostin promotes nucleus pulposus cells apoptosis by activating the Wnt/β-catenin signaling pathway [ FASEB J, 2022, 36(7):e22369] | PubMed: 35747912 |
Novel Insights into the Molecular Regulation of Ribonucleotide Reductase in Adrenocortical Carcinoma Treatment [ Cancers (Basel), 2021, 13(16)4200] | PubMed: 34439352 |
The β-catenin/YAP signaling axis is a key regulator of melanoma-associated fibroblasts [ Signal Transduct Target Ther, 2019, 4:63] | PubMed: 31885878 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
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