Isoxazole 9 (ISX-9)

製品コードS7914 バッチS791401

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C11H10N2O2S
分子量 234.27 CAS No. 832115-62-5
Solubility (25°C)* 体外 DMSO 46 mg/mL (196.35 mM)
Ethanol (warmed with 50ºC water bath) 13 mg/mL (55.49 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Isoxazole 9 (Isx-9) is a synthetic promotor of adult neurogenesis by triggering neuronal differentiation of adult neural stem/precursor cells (NSPCs). Isoxazole 9 (Isx-9) activates multiple pathways including TGF-β induced epithelial–mesenchymal transition (EMT) signaling, canonical and non-canonical Wnt signaling at different stages of cardiac differentiation.
in vitro Isoxazole 9 (ISX-9) increases cell number and promotes cell differentiation in NSPCs, whereas it induces cell damage in OPCs. In outgrowth EPCs, this compound decreases tube formation without effect on early EPCs. [1]
in vivo In mice, isoxazole 9 (ISX-9) (20 mg/kg, i.p.) crosses the BBB and increases proliferation of neuroblasts and neurogenesis via Mef2-specific mechanisms in the hippocampal SGZ. This compound also increases differentiation and dendritic complexity of immature neurons and improves memory. In MWM, it improves spatial memory. [2]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 NSPCs, OPCs, early EPCs, and outgrowth EPCs
濃度 ~50 μM
反応時間 48 h
実験の流れ Cells are treated with different concentrations of Isoxazole 9 (ISX-9) (0, 6.25, 12.5, 25, or 50 μM), and 2 days later, cell numbers are counted in a blinded manner to evaluate the effects of this compound on the proliferation/survival of four types of progenitor/precursor cells.
動物実験 動物モデル Transgenic Nkx2.5-luciferase reporter mice
投薬量 20 mg/kg
投与方法 i.p.

参考

  • https://pubmed.ncbi.nlm.nih.gov/26407349/
  • https://pubmed.ncbi.nlm.nih.gov/22542682/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Signal transduction pathways controlling Ins2 gene activity and beta cell state transitions [ iScience, 2025, 28(3):112015] PubMed: 40144638
Characterization and neurogenic responses of primary and immortalized Müller glia [ Front Cell Dev Biol, 2025, 13:1513163] PubMed: 40417181
The critical role of BMP signaling in gastric epithelial cell differentiation revealed by organoids [ Cell Regen, 2025, 14(1):18] PubMed: 40377813
Direct reprogramming of fibroblasts into spiral ganglion neurons by defined transcription factors [ Cell Prolif, 2024, e13775.] PubMed: 39551613
Short Peptides Protect Fibroblast-Derived Induced Neurons from Age-Related Changes [ Int J Mol Sci, 2024, 25(21)11363] PubMed: 39518916
ISX-9 potentiates CaMKIIδ-mediated BMAL1 activation to enhance circadian amplitude [ Commun Biol, 2022, 5(1):750] PubMed: 35902736
Cochlear Sox2+ Glial Cells Are Potent Progenitors for Spiral Ganglion Neuron Reprogramming Induced by Small Molecules [ Front Cell Dev Biol, 2021, 9:728352] PubMed: 34621745
Cochlear Sox2+ Glial Cells Are Potent Progenitors for Spiral Ganglion Neuron Reprogramming Induced by Small Molecules [ Front Cell Dev Biol, 2021, 9:728352] PubMed: 34621745

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。