受注:045-509-1970 |
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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder | ||||||||
化学式 | C22H24Cl2N2O4
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分子量 | 451.34 | CAS No. | 548470-11-7 | ||||||||
Solubility (25°C)* | 体外 | DMSO | 10 mg/mL (22.15 mM) | ||||||||
Water | Insoluble | ||||||||||
Ethanol | Insoluble | ||||||||||
体内 (毎回新しく調製した物を用意してください) |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | ISRIB is an integrated stress response (ISR) inhibitor that potently reverses the effects of eukaryotic initiation factor 2α (eIF2α) phosphorylation. |
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in vitro | ISRIB, an integrated stress response (ISR) inhibitor, reverses the effect of thapsigargin (TG) on translation, notably inhibits ER stress-induced cFLIP loss and prevents cFLIP levels down-regulation.[2] |
in vivo | In an instability-induced intervertebral disc degeneration (IDD) model in mice, ISRIB suppressed p-eIF2α/ATF4/Ihh and prevented IDD via protecting the anti-oxidative enzymes and decreased the NP cell apoptosis.[3] |
細胞アッセイ | 細胞株 | HCT116 cells |
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濃度 | 220 nM | |
反応時間 | 1 h | |
実験の流れ | HCT116 cells are pre-treated with ISRIB (220 nM) for 1 h prior TG treatment for the indicated times. To analyze protein synthesis, puromycin is added in the last 10 min of treatment and puromycin incorporation to the nascent protein chain is assessed by western blotting using an anti-puromycin antibody. Levels of both cFLIP isoforms are determined in whole cell extracts by western blotting and quantified. |
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動物実験 | 動物モデル | C57 background BL/6J mice of intervertebral disc degeneration (IDD) models |
投薬量 | 0.25 mg/kg | |
投与方法 | s.c. |
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NAD+ dependent UPRmt activation underlies intestinal aging caused by mitochondrial DNA mutations [ Nat Commun, 2024, 15(1):546] | PubMed: 38228611 |
Nucleus pulposus cells regulate macrophages in degenerated intervertebral discs via the integrated stress response-mediated CCL2/7-CCR2 signaling pathway [ Exp Mol Med, 2024, 56(2):408-421.] | PubMed: 38316963 |
Deubiquitylase OTUD3 regulates integrated stress response to suppress progression and sorafenib resistance of liver cancer [ Cell Rep, 2024, 43(7):114487] | PubMed: 38996071 |
GDF15 ameliorates sepsis-induced lung injury via AMPK-mediated inhibition of glycolysis in alveolar macrophage [ Respir Res, 2024, 25(1):201] | PubMed: 38725041 |
Growth hormone resistance induced by amino acid deprivation in fao cells is independent of FGF21 [ Biochem Biophys Res Commun, 2024, 709:149811] | PubMed: 38569244 |
ERK mediates interferon gamma-induced melanoma cell death [ Mol Cancer, 2023, 10.1186/s12943-023-01868-x] | PubMed: 37803324 |
Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice [ Nat Commun, 2023, 14(1):1521] | PubMed: 36934083 |
E3 ligase HECTD3 promotes RNA virus replication and virus-induced inflammation via K33-linked polyubiquitination of PKR [ Cell Death Dis, 2023, 14(7):396] | PubMed: 37402711 |
E3 ligase HECTD3 promotes RNA virus replication and virus-induced inflammation via K33-linked polyubiquitination of PKR [ Cell Death Dis, 2023, 14(7):396] | PubMed: 37402711 |
ETHE1 Accelerates Triple-Negative Breast Cancer Metastasis by Activating GCN2/eIF2α/ATF4 Signaling [ Int J Mol Sci, 2023, 24(19)14566] | PubMed: 37834012 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。