ISRIB

製品コードS0706 バッチS070603

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
化学式
C22H24Cl2N2O4
分子量 451.34 CAS No. 548470-11-7
Solubility (25°C)* 体外 DMSO 8 mg/mL (17.72 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 ISRIB is an integrated stress response (ISR) inhibitor that potently reverses the effects of eukaryotic initiation factor 2α (eIF2α) phosphorylation.
in vitro

ISRIB, an integrated stress response (ISR) inhibitor, reverses the effect of thapsigargin (TG) on translation, notably inhibits ER stress-induced cFLIP loss and prevents cFLIP levels down-regulation.[2]
in vivo

In an instability-induced intervertebral disc degeneration (IDD) model in mice, ISRIB suppressed p-eIF2α/ATF4/Ihh and prevented IDD via protecting the anti-oxidative enzymes and decreased the NP cell apoptosis.[3]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HCT116 cells
濃度 220 nM
反応時間 1 h
実験の流れ

HCT116 cells are pre-treated with ISRIB (220 nM) for 1 h prior TG treatment for the indicated times. To analyze protein synthesis, puromycin is added in the last 10 min of treatment and puromycin incorporation to the nascent protein chain is assessed by western blotting using an anti-puromycin antibody. Levels of both cFLIP isoforms are determined in whole cell extracts by western blotting and quantified.
動物実験 動物モデル C57 background BL/6J mice of intervertebral disc degeneration (IDD) models
投薬量 0.25 mg/kg
投与方法 s.c.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

NAD+ dependent UPRmt activation underlies intestinal aging caused by mitochondrial DNA mutations [ Nat Commun, 2024, 15(1):546] PubMed: 38228611
Nucleus pulposus cells regulate macrophages in degenerated intervertebral discs via the integrated stress response-mediated CCL2/7-CCR2 signaling pathway [ Exp Mol Med, 2024, 56(2):408-421.] PubMed: 38316963
ERK mediates interferon gamma-induced melanoma cell death [ Mol Cancer, 2023, 10.1186/s12943-023-01868-x] PubMed: 37803324
Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice [ Nat Commun, 2023, 14(1):1521] PubMed: 36934083
E3 ligase HECTD3 promotes RNA virus replication and virus-induced inflammation via K33-linked polyubiquitination of PKR [ Cell Death Dis, 2023, 14(7):396] PubMed: 37402711
E3 ligase HECTD3 promotes RNA virus replication and virus-induced inflammation via K33-linked polyubiquitination of PKR [ Cell Death Dis, 2023, 14(7):396] PubMed: 37402711
ETHE1 Accelerates Triple-Negative Breast Cancer Metastasis by Activating GCN2/eIF2α/ATF4 Signaling [ Int J Mol Sci, 2023, 24(19)14566] PubMed: 37834012
Neuron-specific translational control shift ensures proteostatic resilience during ER stress [ EMBO J, 2022, e110501] PubMed: 35791631
cFLIP downregulation is an early event required for endoplasmic reticulum stress-induced apoptosis in tumor cells [ Cell Death Dis, 2022, 13(2):111] PubMed: 35115486
Endoplasmic reticulum oxidoreductin 1-alpha deficiency and activation of protein translation synergistically impair breast tumour resilience [ Br J Pharmacol, 2022, 179(23):5180-5195] PubMed: 35853086

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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