ITD-1

製品コードS6713 バッチS671302

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₂₇H₂₉NO₃

分子量

415.52

CAS No.

1099644-42-4

Solubility (25°C)*

体外

DMSO

83 mg/mL (199.74 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	5.000mg/ml (12.03mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	5.000mg/ml (12.03mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	ITD-1 is a potent TGF-β inhibitor. It does not block the kinase activity of either type I (TGFBR1) or type II (TGFBR2) TGFβ receptors but potently blocks phosphorylation of the effector SMAD2/3 proteins induced by TGFβ2, and only minimally in response to Activin A.
in vitro	ITD-1, a small molecule inhibitor of TGF-β signaling, can partially induce proteasomal degradation of the TβR232,33, and blocks Smad and MAPK activation.^[2]
in vivo	ITD-1 partially inhibits TGF-β signaling and renal fibrosis in unilateral ischemia-reperfusion injury (UIRI) mice.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	NRK-49F cells
	濃度	3 μM
	反応時間	1 h
	実験の流れ	NRK-49F cells are pre-incubated with KP1 (10 µg/ml, 3 μM) or ITD-1 (3 μM) for 1 h, and then treated with TGF-β1 (2 ng/ml) for 45 min. Western blot analyses the protein levels of p-Smad3 and p-p38.
動物実験	動物モデル	unilateral ischemia-reperfusion injury (UIRI) mice, unilateral ureter obstruction (UUO) mice
	投薬量	0.31 μM/kg
	投与方法	IOCV

参考

https://pubmed.ncbi.nlm.nih.gov/22862949/
https://pubmed.ncbi.nlm.nih.gov/35064106/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Interleukin-17A facilitates tumor progression via upregulating programmed death ligand-1 expression in hepatocellular carcinoma [ World J Gastrointest Oncol, 2025, 17(1):97831]	PubMed: 39817127
UBR5 mediates colorectal cancer chemoresistance by attenuating ferroptosis via Lys 11 ubiquitin-dependent stabilization of Smad3-SLC7A11 signaling [ Redox Biol, 2024, 76:103349]	PubMed: 39260061
Low-dose SAHA enhances CD8+ T cell-mediated antitumor immunity by boosting MHC I expression in non-small cell lung cancer [ Cell Oncol (Dordr), 2024, 10.1007/s13402-024-00989-9]	PubMed: 39283477
Alpha-hederin reprograms multi-miRNAs activity and overcome small extracellular vesicles-mediated paclitaxel resistance in NSCLC [ Front Pharmacol, 2024, 15:1257941]	PubMed: 38362150
Adipose stem cells-derived small extracellular vesicles transport Thrombospondin 1 cargo to promote insulin resistance in gestational diabetes mellitus [ Diabetol Metab Syndr, 2024, 16(1):105]	PubMed: 38764083
KIAA1429-mediated m6A modification of CHST11 promotes progression of diffuse large B-cell lymphoma by regulating Hippo-YAP pathway [ Cell Mol Biol Lett, 2023, 28(1):32]	PubMed: 37076815
Tripartite motif-containing 9 promoted proliferation and migration of bladder cancer cells through CEACAM6-Smad2/3 axis [ J Cell Commun Signal, 2023, 10.1007/s12079-023-00766-7]	PubMed: 37249822
A Klotho-derived peptide protects against kidney fibrosis by targeting TGF-β signaling [ Nat Commun, 2022, 13(1):438]	PubMed: 35064106
TGF-β induces GBM mesenchymal transition through upregulation of CLDN4 and nuclear translocation to activate TNF-α/NF-κB signal pathway [ Cell Death Dis, 2022, 13(4):339]	PubMed: 35418179
HOXA10 mediates epithelial-mesenchymal transition to promote gastric cancer metastasis partly via modulation of TGFB2/Smad/METTL3 signaling axis [ J Exp Clin Cancer Res, 2021, 40(1):62]	PubMed: 33563300

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。