IWP-2

製品コードS7085 バッチS708505

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₂₂H₁₈N₄O₂S₃
	分子量	466.6	CAS No.	686770-61-6
Solubility (25°C)*	体外	DMF	20 mg/mL warmed with 50ºC water bath (42.86 mM)
		DMSO	Insoluble
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	IWP-2 is an inhibitor of Wnt processing and secretion with IC50 of 27 nM in a cell-free assay, selective blockage of Porcn-mediated Wnt palmitoylation, does not affect Wnt/β-catenin in general and displays no effect against Wnt-stimulated cellular responses. IWP-2 specifically inhibits CK1δ.
in vitro	IWP-2 is useful in both regenerative medicine and anticancer efforts. IWP-2 inactivates Porcn, a membrane-bound O-acyltransferase (MBOAT), and selectively inhibits palmitoylation of Wnt. IWP-2 blocks Wnt-dependent phosphorylation of Lrp6 receptor and Dvl2, and β-catenin accumulation. ^[1]
特徴	Selective inhibitor of Porcn-mediated Wnt secretion.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	L-Wnt-STF cells
	濃度	5 μM
	反応時間	24 h
	実験の流れ	L-Wnt-STF cells that exhibit constitutive Wnt pathway activation were incubated with IWR (10μM) and IWP (5μM) compounds for 24 hrs prior to lysis. Cellular lysates were subjected to Western blot analysis.
動物実験	動物モデル	Crl:CD1 (ICR) and FVB/N female mice
	投薬量	50 µg
	投与方法	s.c.

参考

カスタマーフィードバック

: Data from [Data independently produced by , , Sci Rep, 2016, 6:32770.]

: Data from [Data independently produced by , , Methods, 2016, 101:103-12.]

: Data from [Data independently produced by , , Biochem Biophys Res Commun, 2017, 486(1):49-56]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells [ Nat Commun, 2024, 15(1):1351]	PubMed: 38355589
Derivation of human primordial germ cell-like cells in an embryonic-like culture [ Nat Commun, 2024, 15(1):167]	PubMed: 38167821
Biosafe cerium oxide nanozymes protect human pluripotent stem cells and cardiomyocytes from oxidative stress [ J Nanobiotechnology, 2024, 22(1):132]	PubMed: 38532378
Induced pluripotent stem cell (iPSC) modeling validates reduced GBE1 enzyme activity due to a novel variant, p.Ile694Asn, found in a patient with suspected glycogen storage disease IV [ Mol Genet Metab Rep, 2024, 39:101069]	PubMed: 38516405
Dissecting peri-implantation development using cultured human embryos and embryo-like assembloids [ Cell Res, 2023, 10.1038/s41422-023-00846-8]	PubMed: 37460804
Inhibition of Wnt activity improves peri-implantation development of somatic cell nuclear transfer embryos [ Natl Sci Rev, 2023, 10(9):nwad173]	PubMed: 37593113
Inhibition of Wnt activity improves peri-implantation development of somatic cell nuclear transfer embryos [ Natl Sci Rev, 2023, 10(9):nwad173]	PubMed: 37593113
Spatially controlled construction of assembloids using bioprinting [ Nat Commun, 2023, 14(1):4346]	PubMed: 37468483
Diagnosis of acute myocardial infarction using a combination of circulating circular RNA cZNF292 and clinical information based on machine learning [ MedComm (2020), 2023, 4(3):e299]	PubMed: 37323876
Chemical-induced epigenome resetting for regeneration program activation in human cells [ Cell Rep, 2023, 42(6):112547]	PubMed: 37224020

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。