IWP-2

製品コードS7085 バッチS708505

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₂H₁₈N₄O₂S₃

分子量

466.6

CAS No.

686770-61-6

Solubility (25°C)*

体外

DMF (warmed with 50ºC water bath)

20 mg/mL (42.86 mM)

DMSO (warmed with 50ºC water bath)

3 mg/mL (6.42 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	IWP-2 is an inhibitor of Wnt processing and secretion with IC50 of 27 nM in a cell-free assay, selective blockage of Porcn-mediated Wnt palmitoylation, does not affect Wnt/β-catenin in general and displays no effect against Wnt-stimulated cellular responses. IWP-2 specifically inhibits CK1δ.
in vitro	IWP-2 is useful in both regenerative medicine and anticancer efforts. This compound inactivates Porcn, a membrane-bound O-acyltransferase (MBOAT), and selectively inhibits palmitoylation of Wnt. It blocks Wnt-dependent phosphorylation of Lrp6 receptor and Dvl2, and β-catenin accumulation. ^[1]
特徴	Selective inhibitor of Porcn-mediated Wnt secretion.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	L-Wnt-STF cells
	濃度	5 μM
	反応時間	24 h
	実験の流れ	L-Wnt-STF cells that exhibit constitutive Wnt pathway activation were incubated with IWR (10μM) and IWP-2 (5μM) compounds for 24 hrs prior to lysis. Cellular lysates were subjected to Western blot analysis.
動物実験	動物モデル	Crl:CD1 (ICR) and FVB/N female mice
	投薬量	50 µg
	投与方法	s.c.

参考

https://pubmed.ncbi.nlm.nih.gov/19125156/
https://pubmed.ncbi.nlm.nih.gov/25536657/
https://pubmed.ncbi.nlm.nih.gov/29630366/

カスタマーフィードバック

: Data from [Data independently produced by , , Sci Rep, 2016, 6:32770.]

: Data from [Data independently produced by , , Methods, 2016, 101:103-12.]

: Data from [Data independently produced by , , Biochem Biophys Res Commun, 2017, 486(1):49-56]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Persistent Wnt signaling affects IVF embryo implantation and offspring metabolism [ Sci Bull (Beijing), 2025, S2095-9273(25)00472-4]	PubMed: 40441968
ETVs dictate hPSC differentiation by tuning biophysical properties [ Nat Commun, 2025, 16(1):1999]	PubMed: 40011454
Deciphering signaling mechanisms and developmental dynamics in extraembryonic mesoderm specification from hESCs [ Nat Commun, 2025, 16(1):4688]	PubMed: 40399284
AAV vectors trigger DNA damage response-dependent pro-inflammatory signalling in human iPSC-derived CNS models and mouse brain [ Nat Commun, 2025, 16(1):3694]	PubMed: 40251179
Bioimpedance and electrophysiology measurements of engineered heart tissues outside the culture medium [ Biosens Bioelectron, 2025, 287:117634]	PubMed: 40614400
Zbtb16 determines the fate plasticity of cardiovascular progenitors through IGF2BP3-mediated mRNA stabilization [ Cell Rep, 2025, 44(8):116127]	PubMed: 40779394
Allelic bias contributes to heterogeneous phenotypes of NK cell deficiency [ Cell Rep, 2025, 44(8):116102]	PubMed: 40768335
The balance between IFN-γ and ERK/MAPK signaling activities ensures lifelong maintenance of intestinal stem cells [ Cell Rep, 2025, S2211-1247(25)00057-9]	PubMed: 39952238
Specific induction of right ventricular-like cardiomyocytes from human pluripotent stem cells [ Stem Cell Res Ther, 2025, 16(1):519]	PubMed: 41013775
Directed and efficient generation of calretinin interneurons from human pluripotent stem cells [ Stem Cell Res Ther, 2025, 16(1):465]	PubMed: 40867008

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。