JC-1

製品コードS9784 バッチS978401

化学情報

	Synonyms	CBIC2, NK 1420	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₂₅H₂₇Cl₅N₄
	分子量	560.77	CAS No.	3520-43-2
Solubility (25°C)*	体外	DMSO	33 mg/mL (58.84 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	JC-1 (CBIC2, NK 1420), a fluorescent lipophilic carbocyanine dye, is a mitochondrial potential (ΔΨ(m)) marker. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers.
in vitro	1. Take the 6-well plate as an example for cell planking, and the density is 5×10^5/mL. Incubate overnight in 5% CO2 incubator at 37℃. Note: it is suggested that the cell density during apoptosis induction should not exceed 1×10^6/ml, which can also be cultured to the appropriate density according to your own cell type. 2. Take 0.5 mL suspension into sterile centrifuge tube; 400 g centrifugation for 3-5 min; Discard the supernatant. 3. The cells were resuspended with 1mljc-1 working solution and incubated in 5% CO2 incubator at 37℃for 15-30 min. 4. Centrifugation at room temperature for 5 min at 400 g; Suck of the supernatant. 5. The cells were resuspended with 2 mL cell culture medium or buffer, and then centrifuged at room temperature for 5 min at 400 g; Discard the supernatant and repeat twice. 6. Resuspend the cells with 1mL of fresh culture medium or buffer, and immediately conduct subsequent flow cytometry or fluorescence microscope observation. 7. Data analysis (flow cytometry) : mitochondria of healthy cells containing red JC-1 aggregates were detected by FL2 channel; Apoptotic or unhealthy cells containing green JC-1 monomer were detected by FL1 (FITC) channel. 8. If used for enzyme labeling instrument, use 300 μL buffer resuspended cells; Then 100 per hole μ Transfer the stained cells to a light tight 96 well plate with the amount of L, and then conduct fluorescent enzyme label plate analysis.

製品説明

JC-1 (CBIC2, NK 1420), a fluorescent lipophilic carbocyanine dye, is a mitochondrial potential (ΔΨ(m)) marker. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers.

in vitro

1. Take the 6-well plate as an example for cell planking, and the density is 5×10^5/mL. Incubate overnight in 5% CO2 incubator at 37℃.
Note: it is suggested that the cell density during apoptosis induction should not exceed 1×10^6/ml, which can also be cultured to the appropriate density according to your own cell type.
2. Take 0.5 mL suspension into sterile centrifuge tube; 400 g centrifugation for 3-5 min; Discard the supernatant.
3. The cells were resuspended with 1mljc-1 working solution and incubated in 5% CO2 incubator at 37℃for 15-30 min.
4. Centrifugation at room temperature for 5 min at 400 g; Suck of the supernatant.
5. The cells were resuspended with 2 mL cell culture medium or buffer, and then centrifuged at room temperature for 5 min at 400 g; Discard the supernatant and repeat twice.
6. Resuspend the cells with 1mL of fresh culture medium or buffer, and immediately conduct subsequent flow cytometry or fluorescence microscope observation.
7. Data analysis (flow cytometry) : mitochondria of healthy cells containing red JC-1 aggregates were detected by FL2 channel; Apoptotic or unhealthy cells containing green JC-1 monomer were detected by FL1 (FITC) channel.
8. If used for enzyme labeling instrument, use 300 μL buffer resuspended cells; Then 100 per hole μ Transfer the stained cells to a light tight 96 well plate with the amount of L, and then conduct fluorescent enzyme label plate analysis.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	hippocampal neurons and glial cells
	濃度	1 μg/ml
	反応時間	15 min
	実験の流れ	JC-1 is dissolved in dimethylsulfoxide (DMSO) as a 2 mg/ml stock. JC-1 is excited at 490 nm and an optical image splitter device is attached to the microscope to separate spectrally the green and red components of JC-1 fluorescence.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Irisin attenuates sepsis-induced cardiac dysfunction by attenuating inflammation-induced pyroptosis through a mitochondrial ubiquitin ligase-dependent mechanism [ Biomed Pharmacother, 2022, 152:113199]	PubMed: 35653888
Combining an Aurora Kinase Inhibitor and a Death Receptor Ligand/Agonist Antibody Triggers Apoptosis in Melanoma Cells and Prevents Tumor Growth in Preclinical Mouse Models [ Clin Cancer Res, 2015, 21(23):5338-48]	PubMed: 26152738

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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