JSH-23

製品コードS7351 バッチS735101

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C16H20N2
分子量 240.34 CAS No. 749886-87-1
Solubility (25°C)* 体外 DMSO 48 mg/mL (199.71 mM)
Ethanol 20 mg/mL (83.21 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO Corn oil

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 5.000mg/ml (20.80mM) Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 JSH-23 は NF-κB 転写活性阻害剤であり、RAW 264.7 細胞において LPS 刺激 NF-κB 転写活性を 7.1 μM の IC50 値で阻害し、IκB 分解に影響を与えることなく LPS 誘導 NF-κB 核内移行を妨害します。
in vitro JSH-23 inhibits LPS-induced nuclear translocation of NF-κB p65 without affecting IκBα degradation. This compound inhibits LPS-induced apoptotic chromatin condensation, while does not show significant cytotoxic effects on the RAW 264.7 cells at <100 μM. It also decreases NO production and neuronal migration in LPS activated cultures primary cultures from developing mouse cerebellum. Moreover, this chemical augments cisplatin cytotoxicity in ovarian cancer cells with CI values ranging from 0.35 to 0.85.
in vivo JSH-23 (3 mg/kg) significantly reverses the nerve conduction and nerve blood flow deficits by decreasing neuroinflammation and improving antioxidant defence in diabetic rats.

プロトコル(参考用のみ)

キナーゼアッセイ Measurement of NF-κB transcriptional activity
Macrophages RAW 264.7 transfected stably with reporter plasmid of pNF-κB-SEAP-NPT are treated with 1 μg/ml LPS and/or sample for 16 hours. As the reporter, SEAP activity in the cell-free culture media is measured as followed. Single cell-derived stable transfectants are plated in 5 ml of T-25 flask, and the media is decanted 24 h later. At this time, cells are washed twice with phosphate-buffered saline, and incubations are initiated by addition of new media. This compound is added to the culture medium after 24 h of incubations. Aliquots (25 ml) of medium from a control or chemical-treated cultures are taken at 0, 3, 20, 24, 48, and 72 h, heated at 65°C for 5 min to eliminate the alkaline phosphatase activity, and used immediately or stored at -20°C. Mixtures consisting of dilution buffer (25 ml), assay buffer (97 ml), culture media (25 ml), and 4-methylumbelliferyl phosphate (MUP, 1 mM, 3 ml) in each well of the 96-well plates are incubated for 60 min in the dark at room temperature. Fluorescence emits the product of the SEAP/MUP is measured at 449 nm using a 96-well plate fluorometer after excitation at 360 nm.
細胞アッセイ 細胞株 RAW 264.7 cells
濃度 ~300 μM
反応時間 24 hours
実験の流れ Macrophages RAW 264.7 are incubated with various concentrations of JSH-23 for 24 h. The cells are treated with WST-1 solution and absorbance is measured at 450 nm.
動物実験 動物モデル STZ-induced diabetic rats
投薬量 ~3 mg/kg
投与方法 Oral administration

参考

  • https://pubmed.ncbi.nlm.nih.gov/15280016/
  • https://pubmed.ncbi.nlm.nih.gov/20955790/
  • https://pubmed.ncbi.nlm.nih.gov/24418212/
  • https://pubmed.ncbi.nlm.nih.gov/21447040/

カスタマーフィードバック

Data from [Data independently produced by , , Cell, 2018, 172(4):841-856]

Data from [Data independently produced by , , Blood, 2015, 125(7): 1098-106 ]

Data from [Data independently produced by , , J Mol Med (Berl), 2018, 96(3-4):301-313]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Viruses hijack FPN1 to disrupt iron withholding and suppress host defense [ Nat Commun, 2025, 16(1):5912] PubMed: 40595467
Heterogeneous Activation of Signaling Pathways and Therapeutic Vulnerabilities in KSHV-Associated Primary Effusion Lymphoma Cell Lines [ J Med Virol, 2025, 97(8):e70534] PubMed: 40751690
Tumor-derived exosomes induce neutrophil infiltration and reprogramming to promote T-cell exhaustion in hepatocellular carcinoma [ Theranostics, 2025, 15(7):2852-2869] PubMed: 40083930
Yttrium nitrate activates the oxidative stress-mediated NF-κB pathway to induce testicular inflammatory response and reduce sperm quality in mice [ Ecotoxicol Environ Saf, 2025, 302:118720] PubMed: 40706520
Targeting synergetic endothelial inflammation by inhibiting NFKB and JAK-STAT pathways [ iScience, 2025, 28(9):113307] PubMed: 40894883
DPEP2 suppresses metastasis via NF-κB-mediated epithelial-mesenchymal transition and M2 polarization in p53-loss non-small cell lung cancer [ Cancer Cell Int, 2025, 25(1):408] PubMed: 41250029
CpG-oligodeoxynucleotides challenged macrophages ameliorate acetaminophen induced liver injury by activating TLR9/IRG1/itaconate metabolic pathway [ Mol Med, 2025, 31(1):282] PubMed: 40855408
Regulator of G protein signaling-1 facilitates ovarian cancer development by modulating NF-kB signal pathway [ Sci Rep, 2025, 15(1):864] PubMed: 39757280
Copper-mediated MAM regulation of the NF-κB signalling pathway enhances Seneca Valley virus replication in PK-15 cells [ Vet Res, 2025, 56(1):175] PubMed: 40973945
Proteomic and network pharmacology analyses reveal S100A8 as the anti-inflammatory target of Yunpi Jiedu Tongluo Qushi Granule in the treatment of rheumatoid arthritis [ J Pharm Biomed Anal, 2025, 252:116522] PubMed: 39442465

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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