JSH-23

製品コードS7351 バッチS735101

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C16H20N2
分子量 240.34 CAS No. 749886-87-1
Solubility (25°C)* 体外 DMSO 48 mg/mL (199.71 mM)
Ethanol 20 mg/mL (83.21 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO Corn oil

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 5.000mg/ml (20.80mM) Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 JSH-23 is an inhibitor of NF-κB transcriptional activity, which inhibits LPS-stimulated nuclear factor (NF)-κB transcriptional activity in RAW 264.7 cells with an IC50 value of 7.1 μM, and interferes with LPS-induced NF-κB nuclear translocation without affecting IκB degradation.
in vitro JSH-23 inhibits LPS-induced nuclear translocation of NF-κB p65 without affecting IκBα degradation. This compound inhibits LPS-induced apoptotic chromatin condensation, while does not show significant cytotoxic effects on the RAW 264.7 cells at <100 μM. [1] It also decreases NO production and neuronal migration in LPS activated cultures primary cultures from developing mouse cerebellum. [2] Moreover, this chemical augments cisplatin cytotoxicity in ovarian cancer cells with CI values ranging from 0.35 to 0.85. [3]
in vivo JSH-23 (3 mg/kg) significantly reverses the nerve conduction and nerve blood flow deficits by decreasing neuroinflammation and improving antioxidant defence in diabetic rats. [4]

プロトコル(参考用のみ)

キナーゼアッセイ Measurement of NF-κB transcriptional activity
Macrophages RAW 264.7 transfected stably with reporter plasmid of pNF-κB-SEAP-NPT are treated with 1 μg/ml LPS and/or sample for 16 hours. As the reporter, SEAP activity in the cell-free culture media is measured as followed. Single cell-derived stable transfectants are plated in 5 ml of T-25 flask, and the media is decanted 24 h later. At this time, cells are washed twice with phosphate-buffered saline, and incubations are initiated by addition of new media. This compound is added to the culture medium after 24 h of incubations. Aliquots (25 ml) of medium from a control or chemical-treated cultures are taken at 0, 3, 20, 24, 48, and 72 h, heated at 65°C for 5 min to eliminate the alkaline phosphatase activity, and used immediately or stored at -20°C. Mixtures consisting of dilution buffer (25 ml), assay buffer (97 ml), culture media (25 ml), and 4-methylumbelliferyl phosphate (MUP, 1 mM, 3 ml) in each well of the 96-well plates are incubated for 60 min in the dark at room temperature. Fluorescence emits the product of the SEAP/MUP is measured at 449 nm using a 96-well plate fluorometer after excitation at 360 nm.
細胞アッセイ 細胞株 RAW 264.7 cells
濃度 ~300 μM
反応時間 24 hours
実験の流れ Macrophages RAW 264.7 are incubated with various concentrations of JSH-23 for 24 h. The cells are treated with WST-1 solution and absorbance is measured at 450 nm.
動物実験 動物モデル STZ-induced diabetic rats
投薬量 ~3 mg/kg
投与方法 Oral administration

参考

  • https://pubmed.ncbi.nlm.nih.gov/15280016/
  • https://pubmed.ncbi.nlm.nih.gov/20955790/
  • https://pubmed.ncbi.nlm.nih.gov/24418212/
  • https://pubmed.ncbi.nlm.nih.gov/21447040/

カスタマーフィードバック

Data from [Data independently produced by , , Cell, 2018, 172(4):841-856]

Data from [Data independently produced by , , Blood, 2015, 125(7): 1098-106 ]

Data from [Data independently produced by , , J Mol Med (Berl), 2018, 96(3-4):301-313]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Viruses hijack FPN1 to disrupt iron withholding and suppress host defense [ Nat Commun, 2025, 16(1):5912] PubMed: 40595467
Tumor-derived exosomes induce neutrophil infiltration and reprogramming to promote T-cell exhaustion in hepatocellular carcinoma [ Theranostics, 2025, 15(7):2852-2869] PubMed: 40083930
Yttrium nitrate activates the oxidative stress-mediated NF-κB pathway to induce testicular inflammatory response and reduce sperm quality in mice [ Ecotoxicol Environ Saf, 2025, 302:118720] PubMed: 40706520
Targeting synergetic endothelial inflammation by inhibiting NFKB and JAK-STAT pathways [ iScience, 2025, 28(9):113307] PubMed: 40894883
CpG-oligodeoxynucleotides challenged macrophages ameliorate acetaminophen induced liver injury by activating TLR9/IRG1/itaconate metabolic pathway [ Mol Med, 2025, 31(1):282] PubMed: 40855408
Regulator of G protein signaling-1 facilitates ovarian cancer development by modulating NF-kB signal pathway [ Sci Rep, 2025, 15(1):864] PubMed: 39757280
Copper-mediated MAM regulation of the NF-κB signalling pathway enhances Seneca Valley virus replication in PK-15 cells [ Vet Res, 2025, 56(1):175] PubMed: 40973945
Proteomic and network pharmacology analyses reveal S100A8 as the anti-inflammatory target of Yunpi Jiedu Tongluo Qushi Granule in the treatment of rheumatoid arthritis [ J Pharm Biomed Anal, 2025, 252:116522] PubMed: 39442465
Nlrc3 signaling is indispensable for hematopoietic stem cell emergence via Notch signaling in vertebrates [ Nat Commun, 2024, 15(1):226] PubMed: 38172511
Dietary vitamin B3 supplementation induces the antitumor immunity against liver cancer via biased GPR109A signaling in myeloid cell [ Cell Rep Med, 2024, 5(9):101718] PubMed: 39293389

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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