受注:045-509-1970 |
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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C20H20N2O4 |
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分子量 | 352.38 | CAS No. | 1431985-92-0 | |
Solubility (25°C)* | 体外 | DMSO | 70 mg/mL (198.64 mM) | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | K02288 is a potent, and selective type I BMP receptor inhibitor with IC50 of 1.1, 1.8, 6.4 nM for ALK2, ALK1 and ALK6, showing weaker inhibition on other ALKs (3, 4, 5) and ActRIIA. |
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in vitro | K02288 dose-dependently inhibits BMP4- and BMP6- induced Smad1/5/8 phosphorylation in C2C12 cells without affecting TGF-β-BMP signaling. [1] |
in vivo | In Zebrafish Embryos, K02288 induces a dorsalized phenotype in a dose dependent manner without effect om intersomitic vessel (ISV) formation. [1] |
特徴 | A chemical probe for BMP signaling in cells. |
キナーゼアッセイ | In vitro Kinase Assay for ALK1-6 | |
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Kinase reactions for ALK1-6 are performed at room temperature for 45 minutes in 96-well plates mixing 2.5 nM kinase, 0.5 mg/mL dephosphorylated casein, 6 µM ATP, 0.05 µCi/µL [γ-32P]ATP, 10 mM MnCl2 and 0.2% BSA in kinase buffer. Inhibitors are added at concentrations between 0 and 10 µM in kinase reaction buffer and tested in triplicate. Reactions are quenched with phosphoric acid, bound to 96-well P81 phosphocellulose filter plates and assayed with Microscint 20 scintillation fluid using a Spectramax L luminometer. Data are normalized to untreated controls at 100% enzyme activity and negative controls subtracted as background. IC50 values are calculated using GraphPad. | ||
動物実験 | 動物モデル | Zebrafish Embryos |
投薬量 | ~10 µM | |
投与方法 | -- |
, , Cancer Invest, 2017, 35(9):601-609
Defects of the spliceosomal gene SNRPB affect osteo- and chondro-differentiation [ FEBS J, 2024, 291(2):272-291] | PubMed: 37584444 |
Inhibition of BMP signaling pathway induced senescence and calcification in anaplastic meningioma [ J Neurooncol, 2024, 10.1007/s11060-024-04625-2] | PubMed: 38446374 |
BMP9-ID1 Signaling Activates HIF-1α and VEGFA Expression to Promote Tumor Angiogenesis in Hepatocellular Carcinoma [ Int J Mol Sci, 2022, 23(3)1475] | PubMed: 35163396 |
Low-Dose-Rate Radiation-Induced Secretion of TGF-β3 Together with an Activator in Small Extracellular Vesicles Modifies Low-Dose Hyper-Radiosensitivity through ALK1 Binding [ Int J Mol Sci, 2022, 23(15)8147] | PubMed: 35897723 |
Electrochemically induced in vitro focal hypoxia in human neurons [ Front Cell Dev Biol, 2022, 10:968341] | PubMed: 36247014 |
Establishment and characterization of immortalized sweat gland myoepithelial cells [ Sci Rep, 2022, 12(1):7] | PubMed: 34997030 |
Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils [ Nat Commun, 2021, 12(1):6177] | PubMed: 34702814 |
BMP9-ID1 signaling promotes EpCAM-positive cancer stem cell properties in hepatocellular carcinoma [ Mol Oncol, 2021, 10.1002/1878-0261.12963] | PubMed: 33834612 |
Hemodynamic Forces Regulate Cardiac Regeneration-Responsive Enhancer Activity during Ventricle Regeneration [ Int J Mol Sci, 2021, 22(8)3945] | PubMed: 33920448 |
GATA3 Mediates a Fast, Irreversible Commitment to BMP4-Driven Differentiation in Human Embryonic Stem Cells. [ Cell Stem Cell, 2020, 7;26(5):693-706] | PubMed: 32302522 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。