Kaempferide

製品コードS3879 バッチS387901

印刷

化学情報

Chemical Structure Synonyms 4'-Methylkaempferol, 4'-O-Methylkaempferol, Kaempferol 4'-methyl ether Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C16H12O6
分子量 300.26 CAS No. 491-54-3
Solubility (25°C)* 体外 DMSO 60 mg/mL (199.82 mM)
Ethanol 13 mg/mL (43.29 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Kaempferide (4'-Methylkaempferol, 4'-O-Methylkaempferol, Kaempferol 4'-methyl ether), a natural compound derived from the roots of kaempferia galanga, has a variety of effects including anti-carcinogenic, anti-inflammatory, anti-oxidant, anti-bacterial and anti-viral properties.
in vitro Kaempferide is able to induce apoptosis in the "side population" (SP, a subpopulation enriched with CSC in various cancers) cells in myeloma. It reverses the activity of drug efflux transporter[2]. Kaempferide is non-toxic to normal fibroblasts while inducing morphological changes, membrane flip-flop and nuclear membrane damage, characteristic of apoptosis in HeLa cells. It is highly cytotoxic to cervical cancer cells. Kaempferide-induced cytotoxicity is independent of cell cycle arrest[3].
in vivo Kaempferide (Kae) remarkably improves cardiac function, alleviates myocardial injury via a decrease in myocardial enzyme levels, and attenuates myocardial infarct size in a dose-dependent manner. It attenuates I/R-induced myocardial injury through inhibition of the Nrf2 and cleaved caspase-3 signaling pathways via a PI3K/Akt/GSK 3β-dependent mechanism. Preconditioning treatment with Kae significantly decreases serum TNF-α, IL-6, C-reactive protein (CRP), MDA, and ROS levels, while increases serum levels of SOD. Nuclear factor erythroid 2-related factor 2 (Nrf2) and cleaved caspase-3 expression levels are downregulated, while phospho-Akt (p-Akt) and phospho-glycogen synthase kinase-3β (p-GSK-3β) expression levels are upregulated[1]. Kaempferide is non-toxic as assessed by acute and chronic toxicity studies in Swiss albino mice in vivo[3].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 KMS-11 cells
濃度 4-64 μM
反応時間 24 h
実験の流れ

Unfractionated cells are seeded at 20000 cells per well in a 96 well plate. After 24h of incubation with various concentration of KFD, PAR, and DMSO (vehicle control), MTS solution is added and absorbance at 490 nm is determined.
動物実験 動物モデル Ischemia/Reperfusion (I/R) Rat Model (Adult Sprague Dawley (SD) rats)
投薬量 0.1 mg/kg, 0.3 mg/kg, 1 mg/kg
投与方法 i.p.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。