Kartogenin

製品コードS7658 バッチS765802

印刷

化学情報

Chemical Structure Synonyms KGN Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C20H15NO3
分子量 317.34 CAS No. 4727-31-5
Solubility (25°C)* 体外 DMSO 63 mg/mL (198.52 mM)
Ethanol (warmed with 50ºC water bath) 5 mg/mL (15.75 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Kartogenin (KGN) is an activator of the smad4/smad5 pathway, and promotes the selective differentiation of multipotent mesenchymal stem cells into chondrocytes.
in vitro Kartogenin induces chondrocyte differentiation from hMSCs by enhancing the nuclear localization of CBFβ, and protects articular chondrocytes. [1] This compound promotes the type-I collagen synthesis of dermal fibroblasts via activation of the smad4/smad5 pathway without obvious cytotoxicity on fibroblasts’ viability, morphology and survival. [2]
in vivo Kartogenin promotes cartilage repair in collagenase VII (10 μM)– and surgery (100 μM)-induced OA models in mice. [1] This compound (100 nM) also stimulats collagen synthesis in the mouse dermis. [2] In addition, it stimulates mouse limb skeletal growth and synovial joint development. [3]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Fibroblasts
濃度 ~1 μM
反応時間 48 hours
実験の流れ For the cell viability assay, fibroblasts are seeded on six-well plates, followed by Kartogenin or DMSO treatment. According to the manufacturer’s instructions, cell viability and viability are assessed by AlamarBlue assay.
動物実験 動物モデル Collagenase VII-induced OA mouse model and Surgery-induced OA mouse model
投薬量 10 μM or 100 μM
投与方法 Intra-articular injection

参考

  • https://pubmed.ncbi.nlm.nih.gov/22491093/
  • https://pubmed.ncbi.nlm.nih.gov/24928394/
  • https://pubmed.ncbi.nlm.nih.gov/25238962/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Enhanced chondrogenic potential in GelMA-based 3D cartilage model via Wnt3a surface immobilization [ Sci Rep, 2024, 14(1):15022] PubMed: 38951570
Fabrication of Injectable Kartogenin-Conjugated Composite Hydrogel with a Sustained Drug Release for Cartilage Repair [ Pharmaceutics, 2023, 15(7)1949] PubMed: 37514135
An Injectable Hydrogel Scaffold Loaded with Dual-Drug/Sustained-Release PLGA Microspheres for the Regulation of Macrophage Polarization in the Treatment of Intervertebral Disc Degeneration [ Int J Mol Sci, 2022, 24(1)390] PubMed: 36613833
Construction of Biocompatible Hydrogel Scaffolds With a Long-Term Drug Release for Facilitating Cartilage Repair [ Front Pharmacol, 2022, 13:922032] PubMed: 35784682
Kartogenin Improves Osteogenesis of Bone Marrow Mesenchymal Stem Cells via Autophagy [ Stem Cells Int, 2022, 2022:1278921] PubMed: 36591373
A chondrogenesis induction system based on a functionalized hyaluronic acid hydrogel sequentially promoting hMSC proliferation, condensation, differentiation, and matrix deposition [ Acta Biomater, 2021, S1742-7061(21)00015-5] PubMed: 33444801
Kartogenin Inhibits Prostate Cancer Cell Growth Through Smad2 Activation and Decreases Androgen Receptor Nuclear Localization [ Anticancer Res, 2021, 41(10):4753-4759] PubMed: 34593424
Intra-articular Injection of Kartogenin-Incorporated Thermogel Enhancing Osteoarthritis Treatment. [ Front Chem, 2019, 7:677] PubMed: 31681730
Effects of kartogenin on the attenuated nucleus pulposus cell degeneration of intervertebral discs induced by interleukin-1β and tumor necrosis factor-α [ Int J Mol Med, 2018, 41(2):749-756] PubMed: 29207013
Retinoic acid and TGF-β signalling cooperate to overcome MYCN-induced retinoid resistance. [Duffy DJ, et al. Genome Med, 2017, 10.1186/s13073-017-0407-3] PubMed: 28187790

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。