Datasheet

生物学的記述

Specificity	Keratin 18 Antibody [M2J10] detects endogenous levels of total Keratin 18 protein.
Background	Keratin 18 (K18) is a type I acidic intermediate filament protein that forms obligate heterodimers with type II keratin 8 (K8) to create the principal cytoskeletal network in simple (single-layered) epithelial tissues such as the liver, pancreas, lung, and gastrointestinal tract. K18 exhibits a canonical tripartite structure, consisting of a glycine/serine-rich N-terminal head domain (with phosphorylation sites Ser33 and Ser52 that regulate filament disassembly), a central α-helical rod domain (~312 amino acids) composed of four coiled-coil segments enabling staggered assembly into heterodimers, tetramers, protofilaments, and 10-nm filaments, and a lysine/arginine-rich C-terminal tail domain for lateral packing. K18/K8 filaments provide mechanical resilience against stress and shear, support organelle positioning (including desmosomes, nucleus, and mitochondria), and are dynamically reorganized during mitosis and apoptosis through caspase-3 cleavage (Asp238 and Asp397) and Ser33 phosphorylation, which can lead to cytoplasmic aggregate formation. K18 also modulates signaling pathways (such as 14-3-3ε, Nox4, and Nrf2) for protection against reactive oxygen species. Clinically, K18 serves as a biomarker for adenocarcinomas (via circulating M30/M65 fragments), and mutations (e.g., p.Ala282Asp, p.Leu127Pro) are linked to filament aggregation, cryptogenic cirrhosis, and increased risk of hepatocellular carcinoma.

Specificity

Keratin 18 Antibody [M2J10] detects endogenous levels of total Keratin 18 protein.

Background

Keratin 18 (K18) is a type I acidic intermediate filament protein that forms obligate heterodimers with type II keratin 8 (K8) to create the principal cytoskeletal network in simple (single-layered) epithelial tissues such as the liver, pancreas, lung, and gastrointestinal tract. K18 exhibits a canonical tripartite structure, consisting of a glycine/serine-rich N-terminal head domain (with phosphorylation sites Ser33 and Ser52 that regulate filament disassembly), a central α-helical rod domain (~312 amino acids) composed of four coiled-coil segments enabling staggered assembly into heterodimers, tetramers, protofilaments, and 10-nm filaments, and a lysine/arginine-rich C-terminal tail domain for lateral packing. K18/K8 filaments provide mechanical resilience against stress and shear, support organelle positioning (including desmosomes, nucleus, and mitochondria), and are dynamically reorganized during mitosis and apoptosis through caspase-3 cleavage (Asp238 and Asp397) and Ser33 phosphorylation, which can lead to cytoplasmic aggregate formation. K18 also modulates signaling pathways (such as 14-3-3ε, Nox4, and Nrf2) for protection against reactive oxygen species. Clinically, K18 serves as a biomarker for adenocarcinomas (via circulating M30/M65 fragments), and mutations (e.g., p.Ala282Asp, p.Leu127Pro) are linked to filament aggregation, cryptogenic cirrhosis, and increased risk of hepatocellular carcinoma.

使用情報

Application

WB, IHC, IF, FCM

Dilution

WB	IHC	IF	FCM
1:2000	1:250 - 1:1000	1:800	1:500 - 1:2000

Reactivity

Human

Source

Mouse Monoclonal Antibody

MW

46 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/34203895/
https://pubmed.ncbi.nlm.nih.gov/19422428/

Application Data

WB

Validated by Selleck

Lane 1: Hela, Lane 2: A431