Kojic acid

製品コードS5174 バッチS517401

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C6H6O4
分子量 142.11 CAS No. 501-30-4
Solubility (25°C)* 体外 DMSO 28 mg/mL (197.03 mM)
Water 25 mg/mL (175.92 mM)
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Kojic acid is a fungal metabolite that inhibits tyrosinase with an IC50 value of 30.6 µM for mushroom tyrosinase. It is a synthetic intermediate used for production of food additives.
in vitro Kojic acid (KA) acts as a good chelator of transition metal ions such as Cu2+ and Fe3+ and a scavenger of free radicals. Kojic acid shows a competitive inhibitory effect on the monophenolase activity and a mixed inhibitory effect on the diphenolase activity of mushroom tyrosinase[1]. Kojic acid could highly protect Chinese hamster ovary (CHO) cells against ionizing radiation with low toxicity[3].
in vivo Kojic acid exerts its radioprotective effects on lethal or sub-lethal irradiation induced damages in mice[3].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 CHO cells
濃度 0, 0.1, 1, 10, 100, 1000, 1500, 2500 μg/ml
反応時間 72 h
実験の流れ CHO cells are incubated in RPMI 1640 medium containing 10% (v/v) fetal bovine serum, and maintained at 37°C in a 5% CO2 incubator. For cytotoxicity assay, CHO cells are seeded in 96-well plates at a density of 2500 cells per well in 180 μl medium. Then, 20 μl of Kojic acid stock solution are added to each well to make sure Kojic acid at the final concentrations of 0, 0.1, 1, 10, 100, 1000, 1500, 2500 μg/ml. After cultured for 72 h, each well of the cells is added with 20 μl MTT (5 mg/mL), which is dissolved in sterile Phosphate Buffered Saline (pH 7.4) and incubated for 4 h at 37°C. Then thesupernatant is discarded and 200 μl DMSO is added to each well. The mixture is sufficiently oscillated in the dark place for 15 min. The optical density values of wells are measured at 492 nm.
動物実験 動物モデル adult C57BL/6 male mice
投薬量 75 or 300 mg/kg
投与方法 s.c.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Inhibition of NAT10 Suppresses Melanogenesis and Melanoma Growth by Attenuating Microphthalmia-Associated Transcription Factor (MITF) Expression. [ Int J Mol Sci, 2017, 18(9)] PubMed: 28880216

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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