L-Kynurenine

製品コードS5839 バッチS583902

化学情報

	Synonyms	(S)-Kynurenine	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₁₀H₁₂N₂O₃
	分子量	208.21	CAS No.	2922-83-0
Solubility (25°C)*	体外	DMSO	21 mg/mL (100.85 mM)
		Water	14 mg/mL (67.23 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	L-Kynurenine ((S)-Kynurenine) is a aryl hydrocarbon receptor agonist. L-Kynurenine is a metabolite of the amino acid L-tryptophan used in the production of niacin and a central compound of the tryptophan metabolism pathway.
in vitro	Kynurenine (Kyn), the tryptophan (Trp) metabolite and an AHR ligand in hepatocytes, accumulates to cellular levels to cause an elevated rate of AHR activation, and stimulates AHR transcriptional activity quickly. An excess production of Kyn via increased cellular IDO1 levels and activity leads to amplified AHR signaling ultimately giving rise to obesity.^[1]
in vivo	L-kynurenine shows a dose-dependent significant neuroprotective effect before the hypoxia-ischemia of rats, and blocks the induction of c-fos immunoreactivity in cerebral cortex following ischemia.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	H1L7.5c3 mouse hepatocytes
	濃度	0.1, 1, 10, 100 μM
	反応時間	24 h
	実験の流れ	H1L7.5c3 cells are seeded in white-walled, white-bottomed 96-well plates at 4000 cells/well and incubated for 24 hr in culture medium. After the 24-hr incubation, the medium is removed, and the cells are washed once with Dulbecco's Phosphate Buffered Saline. Cells are treated with Kynurenine (Kyn) for an additional 24 hr, all of which are dissolved in DMSO unless otherwise noted. Then, cells are removed from incubation and allowed to equilibrate to room temperature for 15min. After equilibration, the medium is removed and the cells are washed twice with at room temperature with DPBS. The cells are lysed with 20 μl/well 1 Passive Lysis Buffer and shaken for 20 min at room temperature. Luciferase activity is recorded programmed to inject 50 μl Luciferase Assay Reagent per well with a 10 sec integration of emitted luminescence.
動物実験	動物モデル	7-day-old rats
	投薬量	100, 200, or 300 mg/kg; 50, 100, 200, 300, 400 mg/kg
	投与方法	i.p.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

A novel lncRNA linc-AhRA negatively regulates innate antiviral response in murine microglia upon neurotropic herpesvirus infection [ Theranostics, 2021, 11(19):9623-9651]	PubMed: 34646390

A novel lncRNA linc-AhRA negatively regulates innate antiviral response in murine microglia upon neurotropic herpesvirus infection [ Theranostics, 2021, 11(19):9623-9651]

PubMed: 34646390

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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