受注:045-509-1970 |
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Synonyms | NG-Nitroarginine methyl ester, N-Nitro-L-arginine methylester | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C7H15N5O4.HCl |
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分子量 | 269.69 | CAS No. | 51298-62-5 | |
Solubility (25°C)* | 体外 | Water | 54 mg/mL (200.22 mM) | |
DMSO | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | L-NAME HCl (NG-Nitroarginine methyl ester, N-Nitro-L-arginine methylester) is a nonselective inhibitor of nitric oxide synthetases (NOS) for nNOS (bovine), eNOS (human), and iNOS (murine), with Ki of 15 nM, 39 nM and 4.4 μM, respectively. |
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in vitro | NG-nitro-L-arginine methyl ester (L-NAME; at 0.1-100 mM) causes concentration-dependent inhibition of the Ca2(+)-dependent endothelial NO synthase from porcine aortae. L-NAME causes an endothelium-dependent contraction and an inhibition of the endothelium-dependent relaxation induced by acetylcholine (ACh) in aortic rings. [2] In another research, Viability of rMC-1 cells or BREC in 25 mM glucose is significantly less than at 5 mM glucose, and this cell death is inhibited by l-NAME in both cell types. [3] |
in vivo | L-NAME (0.03-300 mg kg-1, i.v.) induces a dose-dependent increase in mean systemic arterial blood pressure accompanied by bradycardia. L-NAME (100 mg kg-1, i.v.) inhibits significantly the hypotensive responses to ACh and bradykinin. The increase in blood pressure and bradycardia produced by L-NAME is reversed by L-arginine (30-100 mg kg-1, i.v.) in a dose-dependent manner. [2] |
キナーゼアッセイ | Enzyme Assay | |
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The oxidation of L-arginine is monitored by the conversion of [3H]- or [14C]-arginine to L-citrulline which separates L-citrulline from L-arginine by Dowex 50x8-200 (Na) chromatography. Typical reaction mixtures (100 pL) contains 50 mM HEPES, pH 7.0, 8 pM tetrahydrobiopterin, 1 mM CaC12, 0.01 mg/mL calmodulin, 0.5 mM EDTA, 0.450 pM [14C]-arginine (30000 cpm), and 100-200 pM NADPH. The cNOS-catalyzed oxidation of NADPH to NADP+ is monitored by the reduction of absorbance at 340 nm with a Kontron 860 spectrophotometer in a volume of 300 pL. All reactions are at 30 ℃ unless otherwise indicated. | ||
細胞アッセイ | 細胞株 | rMC-1 cells |
濃度 | 1 mM | |
反応時間 | 5 days | |
実験の流れ | rMC-1 cells are incubated in 5 or 25 mM glucose, with or without l-NAME (1 mM). Media is changed every other day for up to 5 days. BREC cells are incubated in 5 or 25 mM glucose as well as inhibitor as described above for 5 days. Cell death is determined by light microscopy using a hemocytometer and a 0.4% trypan blue dye exclusion method. The number of cells that do not exclude the dye is expressed per 1,000 total cells. A minimum of 800 cells is counted per assay (8 dishes, >100 cells counted per dish), and the assay is replicated three times on different days. | |
動物実験 | 動物モデル | Male Wistar rats |
投薬量 | 100 mg/kg | |
投与方法 | i.v. |
, , Cancer Biol Ther, 2014, 15(6):758-67.
, , Biochem Biophys Res Commun, 2015, 465(4):803-9.
Data from [Data independently produced by , , Front Plant Sci, 2016, 7:1652.]
Ginsenoside Rg1 attenuates diabetic vascular endothelial dysfunction by inhibiting the calpain-1/ROS/PKC-β axis [ Life Sci, 2023, 329:121972] | PubMed: 37482213 |
NMDA receptor activation induces damage of alveolar type II cells and lung fibrogenesis through ferroptosis [ Biochim Biophys Acta Mol Cell Res, 2023, 1870(7):119535] | PubMed: 37451346 |
Critical role of mitogen-inducible gene 6 in restraining endothelial cell permeability to maintain vascular homeostasis [ J Cell Commun Signal, 2023, 17(1):151-165] | PubMed: 36284029 |
Ginsenoside Rg1 ameliorates chronic intermittent hypoxia-induced vascular endothelial dysfunction by suppressing the formation of mitochondrial reactive oxygen species through the calpain-1 pathway [ J Ginseng Res, 2023, 47(1):144-154] | PubMed: 36644390 |
Vitamin C deficiency induces hypoglycemia and cognitive disorder through S-nitrosylation-mediated activation of glycogen synthase kinase 3β [ Redox Biol, 2022, 56:102420] | PubMed: 35969998 |
Suppression of VEGFD expression by S-nitrosylation promotes the development of lung adenocarcinoma [ J Exp Clin Cancer Res, 2022, 41(1):239] | PubMed: 35941690 |
Viperin deficiency promotes dendritic cell activation and function via NF-kappaB activation during Mycobacterium tuberculosis infection [ Inflamm Res, 2022, 10.1007/s00011-022-01638-3] | PubMed: 36315280 |
Protective effect of Astragaloside IV on chronic intermittent hypoxia-induced vascular endothelial dysfunction through the calpain-1/SIRT1/AMPK signaling pathway [ Front Pharmacol, 2022, 13:920977] | PubMed: 35983375 |
Tianma Gouteng Decoction Exerts Pregnancy-Protective Effects Against Preeclampsia via Regulation of Oxidative Stress and NO Signaling [ Front Pharmacol, 2022, 13:849074] | PubMed: 35401241 |
Aldehyde dehydrogenase 2 protects against acute kidney injury by regulating autophagy via Beclin-1 pathway [ JCI Insight, 2021, 138183] | PubMed: 34228649 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
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