Licochalcone A

製品コードS7828 バッチS782801

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C21H22O4
分子量 338.4 CAS No. 58749-22-7
Solubility (25°C)* 体外 DMSO 67 mg/mL (197.99 mM)
Ethanol 67 mg/mL (197.99 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 3.35mg/ml (9.90mM) Taking the 1 mL working solution as an example, add 50 μL of 67 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution
5% DMSO 95% Corn oil

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 0.47mg/ml (1.39mM) Taking the 1 mL working solution as an example, add 50 μL of 9.4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Licochalcone A is an estrogenic flavanoid extracted from licorice root, showing antimalarial, anticancer, antibacterial and antiviral activities. Phase 3.
in vitro Licochalcone A markedly inhibits the in vitro growth of L. major amastigotes in human MDMs and U937 cells. [1] This compound shows antibacterial effects against all gram-positive bacteria tested and especially against all Bacillus spp. Tested with MICs of 2 to 3 micrograms/mL. [3] In CT-26 colon cancer cells, it reduces the cell viability and DNA synthesis. [4] It also interferes with MAPK signaling cascades, initiates ROS generation, induces oxidative stress and consequently causes BGC cell apoptosis. [5]
in vivo In mice infected with L. major, licochalcone A (5 mg/kg, i.p.) completely prevents lesion development. In mice infected with L. donovani, this compound (150 mg/kg, p.o.) results in > 65 and 85% reductions of parasite loads in the liver and the spleen, respectively. [2] In CT-26 cell-inoculated Balb/c mice, this chemical (1 mg/kg, p.o.) inhibits the tumor growth, and alleviates cisplatin-induced nephrotoxicity and hepatotoxicity. [4]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 CT-26 cells
濃度 ~20 μM
反応時間 72 hours
実験の流れ The viability of CT-26 mouse colon cancer cells is determined via a MTT assay. In brief, colon cancer cells are seeded onto each well of a 96-well plate with DMEM containing 10% FBS and cultured to adhere overnight. The cells are then treated with various concentrations of this compound in serum-free medium for 24 and 72 hr, respectively. Twenty microlitres of a MTT solution (5 mg/ml) is added to each well, and the cells are incubated for 4 hr at 37°. The medium is then removed, and 200 µL of dimethyl sulfoxide is added to each well. The absorbance is determined at 570 nm using a microplate reader.
動物実験 動物モデル Mice infected with L. major
投薬量 5 mg/kg/day
投与方法 i.p. or p.o.

参考

  • https://pubmed.ncbi.nlm.nih.gov/8109916/
  • https://pubmed.ncbi.nlm.nih.gov/8092835/
  • https://pubmed.ncbi.nlm.nih.gov/11959549/
  • https://pubmed.ncbi.nlm.nih.gov/18484961/
  • https://pubmed.ncbi.nlm.nih.gov/25981581/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Licochalcone A protects against LPS-induced inflammation and acute lung injury by directly binding with myeloid differentiation factor 2 (MD2) [ Br J Pharmacol, 2023, 180(8):1114-1131] PubMed: 36480410
Licochalcone a improves cardiac functions after ischemia-reperfusion via reduction of ferroptosis in rats [ Eur J Pharmacol, 2023, 957:176031] PubMed: 37660967
JNK inhibitor IX restrains pancreatic cancer through p53 and p21 [ Front Oncol, 2022, 12:1006131] PubMed: 36568248
Licochalcone A Promotes the Ubiquitination of c-Met to Abrogate Gefitinib Resistance [ Biomed Res Int, 2022, 2022:5687832] PubMed: 35309168
Licochalcone A inhibits cell growth through the downregulation of the Hippo pathway via PES1 in cholangiocarcinoma cells [ Environ Toxicol, 2021, 10.1002/tox.23422] PubMed: 34845814

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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