Ligustrazine hydrochloride

製品コードS3775 バッチS377501

化学情報

	Synonyms	Tetramethylpyrazine HCl, Tetrapyrazine HCl	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₈H₁₂N₂.HCl
	分子量	172.66	CAS No.	76494-51-4
Solubility (25°C)*	体外	DMSO	34 mg/mL (196.91 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Ligustrazine hydrochloride (Tetramethylpyrazine, Tetrapyrazine) is a chemical compound found in natto and in fermented cocoa beans with anti-inflammation, antioxidant, antiplatelet, and antiapoptosis activities.
in vitro	Tetramethylpyrazine (TMP) is described as "calcium antagonist" and produces a vasodilation effect via inhibiting Ca2+ influx and the release of intracellular Ca2+ at first. TMP can affect the calcium influx, at least partly, by mediating the opening of potassium channel. TMP is also reported to have a direct effect on L-type calcium current, since it can reduce calcium transient in a dose-dependent manner when applied to rabbit ventricular myocyte. TMP also exerts an endothelium protective property via downregulating the expression of ICAM-1 and HSP60. TMP can restrain LPS-induced IL-8 overexpression in HUVECs at both the protein and mRNA levels, which is possibly due to blocking the activation of the NF-kB-dependent pathway; the involvement of ERK and p38 MAPK signaling pathway has also been observed^[1].
in vivo	Tetramethylpyrazine (TMP) can stimulate NO production in pulmonary arteries of rat. Akt and the endothelial isoform of nitric oxide synthase (eNOS) phosphorylation were significantly upregulated after TMP pretreatment in vivo. TMP can suppress the proliferation of VSMC in rabbit aortic vascular. TMP can decrease the ANP mRNA expression in cardiomyocyte hypertrophy rat model and suppress the level of pJAK2, pJAK1, or pSTAT3, demonstrating that TMP can inhibit JAK-STAT signal transduction. It has a short in-vivo half-life (T_1/2=2.89 h)^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HL-60 cells
	濃度	300 µg/mL
	反応時間	24, 48, 60, 72 h
	実験の流れ	Cell viability is determined by the MTT assay. HL-60 cells are treated with 300 µg/mL TMP, 0.5 µM As2O3, and 300 µg/mL TMP combined with 0.5 µM As2O3, respectively.
動物実験	動物モデル	A rat model of Parkinson
	投薬量	20 mg/kg/d
	投与方法	i.p.

参考

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。