Lucigenin

製品コードS6824 バッチS682401

化学情報

	Synonyms	NSC-151912, L-6868	Storage (From the date of receipt)	3 years -20°C(in the dark) powder
	化学式	C₂₈H₂₂N₄O₆
	分子量	510.5	CAS No.	2315-97-1
Solubility (25°C)*	体外	DMSO	20 mg/mL (39.17 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Lucigenin (NSC-151912, L-6868) is a fluoerscent probe that exhibits a bluish-green fluorescence in the presence of endogenously generated superoxide anion radicals and chloride in cells.
in vitro	1.1 Preparation of the stock solution Dissolve 1 mg of Lucigenin in 0.1919 mL of DMSO to obtain 10 mM of Lucigenin. Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of Lucigenin working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of Lucigenin working solution. Note: Please adjust the concentration of Lucigenin working solution according to the actual situation. Cell staining 2.1 Cell preparation. For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. 2.2 Add 1 mL of Lucigenin working solution, and then incubate at room temperature for 15 minutes. 2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant. 2.4 Wash twice with PBS, 5 minutes each time. 2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope.

製品説明

Lucigenin (NSC-151912, L-6868) is a fluoerscent probe that exhibits a bluish-green fluorescence in the presence of endogenously generated superoxide anion radicals and chloride in cells.

in vitro

1.1 Preparation of the stock solution
Dissolve 1 mg of Lucigenin in 0.1919 mL of DMSO to obtain 10 mM of Lucigenin.
Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Lucigenin working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of Lucigenin working solution.
Note: Please adjust the concentration of Lucigenin working solution according to the actual situation.
Cell staining
2.1 Cell preparation.
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of Lucigenin working solution, and then incubate at room temperature for 15 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope.

プロトコル(参考用のみ)

参考

[1] Li Y, et al. J Biol Chem. 1998 Jan 23;273(4):2015-23.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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